Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-26 Cooperative journals: 《广西植物》
LIANG Huizi
YANG Jisheng
QIN Yi
PAN Weihu
XIAO Yufei
HUANG Ronglin
WANG Renjie
JIANG Yi
LIU Xiongsheng
Abstract： Hopea chinensis is distributed in China and its seeds are typical stubbornness. In order to investigate the germination adaptability of seeds to temperature and moisture, we carried out an experiment with three different constant temperature (15 ℃, 20 ℃ and 25 ℃) controlled by artificial climate incubators, and six polyethylene glycol (PEG-6000) mass percentage concentrations (0, 5%, 10%, 15%, 25% and 35%) were used to simulate drought stress. The germination characteristics of these seeds were carefully examined. The results were as follows: (1) Temperature exerted a significant influence on seed germination. Regardless of the level of drought stress applied, higher temperatures positively affected the germination rate, germination energy, germination index, radicle length, seeding length and vitality index of seeds, higher temperatures led to shorter germination delay and increased fluctuations in the germination period. (2) Drought stress had a significant impact on seed germination. Under identical temperature conditions, increasing levels of drought stress resulted in reduced germination rate, germination energy, germination index, germination period, radicle length, seeding length and vitality index of seeds. Moreover, higher levels of drought stress led to longer in germination delay of seeds. (3) The interaction between temperature and drought stress had a significant influenced on seed germination. Under drought stress, seed germination exhibited different responses under different temperature conditions. Specifically, under conditions where a PEG-6000 concentration of 35% was applied as drought stress treatment, the germination rates at temperatures of 20 °C and 25 °C were significantly higher than those observed at 15 °C (8.89% and 15.55% compared to lower rates at 15 °C). However, it was important to note that when subjected to both 35% PEG-6000 and a temperature of 15 °C, the seeds no longer germinate. In summary, our results indicate that the suitable temperatures for seed germination are identified as being around 20 °C and 25 °C. It is determined that the most favorable temperature for early seedling growth is 25 °C. As the drought stress intensifies, the inhibitory effect on seed germination become stronger. These findings suggest that temperature plays a positive role in promoting seed germination, while drought stress can significantly hinder this process. Additionally, moderate warming can help alleviate the inhibitory effect of drought stress on seed germination.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-07-05 Cooperative journals: 《广西植物》
Abstract： Liquidambar formosana is an excellent landscape ecological tree species because its beautiful tree shape and red or orange leaves in autumn. In order to understand the genetic basis of discoloration and secondary metabolism of L. formosana leaves, the mixed samples of L. formosana leaves at five discoloration periods were used for full-length transcriptome sequencing using single-molecule real-time sequencing technique (PacBio platform). The results were as follows: (1) High-quality 41.04 Gb data were obtained by full-length transcriptome sequencing, from which 563 180 full-length non-chimeric sequences were identified, and 27 269 high-quality full-length transcripts were obtained by clustering and de-redundancy. In 27 269 full-length transcripts, 2 035 long-chain non-coding RNA (lncRNA) were predicted, and 14 892 simple repeat sequence (SSR) sites and 1 856 transcription factors were detected. (2) The results of gene annotation showed that a total of 24 857 transcripts were annotated in 8 databases such as NR, GO, COG and KEGG, and 124 metabolic pathways were obtained in KEGG database, including ribosome, carbon metabolism, amino acid biosynthesis and so on. 49 and 71 transcripts were involved in flavonoid and chlorophyll metabolism respectively. The above results preliminarily reveal the transcriptome information and functional characteristics of L. formosana leaves during the discoloration period, and provide basic data for the follow-up study of the molecular mechanism of discoloration, the pathway and regulation of pigment metabolism and synthesis, the cloning of related functional genes and the improvement of leaf color.
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