Subjects: Biology >> Biochemistry submitted time 2024-05-25
Abstract： Taking compound nutrient supplements is a common means of health management. No studies have investigated the overall effects of complex nutrient supplements on healthy individuals from the perspective of the urine proteome. In this study, we compared and analyzed the urine proteome of healthy individuals after taking compound nutrient supplements for 2 weeks, 4 weeks and before taking them, and found that the urine proteome changed more significantly after taking compound nutrient supplements for 2 weeks, and the differential proteins and their enriched pathways may be associated with nutrient supplementation, such as erythropoietin receptor (fold change of 449.5 after taking compound nutrient supplements for 2 weeks). The results of this study provide new clues about the health effects of complex nutrient supplements from the perspective of the urine proteome and help to optimize guidelines and recommendations for the use of complex nutrient supplements.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》
XU Pengn
TAN Qiujinn
SONG Haiyunn
ZHENG Shufangn
YANG Xiaozhoun
HUAN Xiujun
ZHANG Taon
ZHOU Chunhengn
WEI Yuanrongn
WANG Wenlinn
Abstract： Macadamia nut (Macadamia integrifolia) is an evergreen nut tree with high economic value. Its kernel is rich in nutrients such as fatty acid and protein, etc. In order to further explore the main regulatory genes related to nutrient formation in M. integrifolia kernels, transcriptomics, gene cloning, fluorescence quantification PCR and bioinformatics techniques were used to screen potential regulatory genes from the kernel transcriptomes of ‘Guire No. 1’ and ‘A4’, which have significantly different nutrient content in M. integrifolia kernels. The results were as follows: (1) Transcriptome analysis showed that 1 667 genes were up-regulated and 1 798 genes down-regulated in ‘Guire No. 1’ kernel compared with those of ‘A4’ kernel and KEGG enrichment analysis showed that the differential genes were mainly in starch and glucose metabolism, amino acid biosynthesis and carbon metabolism. (2) A significant differential gene-LOC122077931 encoding the R2R3-MYB transcription factor MYB44L was discovered. The MiMYB44L gene was cloned in kernels of M. integrifolia variety ‘Guire No. 1’ using RACE technology, which was 1 165 bp in length, 999 bp in ORF in length, and encoded 332 amino acids. (3) Bioinformatics analysis confirmed the presence of the SANT domain in the MiMYB44L protein, a hallmark feature of the R2R3-MYB family. The protein lacked both a signal peptide and a transmembrane domain but featured phosphorylation sites. (4) The protein content in kernels of 10 M. integrifolia varieties was determined. And it was found that the expression of MiMYB44L gene in M. integrifolia varieties with high protein content was significantly higher than that in varieties with low protein content, and the overall correlation coefficient was 0.54, reaching a very significant level. The results of this study provide theoretical guidance for in-depth analysis of the regulatory mechanism of MiMYB44L gene in the formation of protein content in M. integrifolia.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》
Li Jinghan
Zhang Shunwei
Yang Xiaobo
Li Donghai
Qi Chunlin
Huang Yao
Zhang Xiang
Hao Jiewei
Liang Caiqun
Zhu Zicheng
Jiang Yuexin
Wang Chongyang
HE Yiqi
Abstract： Based on the ‘National Key Protected Wild Plants List’ and related data in 2021, combined with field investigation, the ‘National Key Protected Wild Plants List of Hainan Province’ was integrated, and its composition and floristic characteristics were analyzed. The results were as follows: (1) Among the national key protected wild plants, the original Cycas changjiangensis, C. hainanensis, C. lingshuigensis and C. taiwaniana were incorporated into C. taiwaniana; compared with the National Key Protected Wild Plant List in 1999, the original Semiliquidambar cathayensis and Amphicarpaea linearis were removed from the protection category, and Bretschneidera sinensis was degraded from the Category I protection to the Category II protection.127 species of national key protected wild plants were added, and a total of 173 species ( including varieties ) were added, belonging to 53 families and 83 genera, including 8 species of national Category I protected wild plants. There are 165 species of national Category II protected wild plants and 32 endemic species in Hainan Province, which belong to 4 groups. Among them, the angiosperm species are the most abundant, with 129 species. (2) Based on years of field investigation information and the results of this survey, Cycas rumphii, C. shanyagensis, Alsophila costularis and Paphiopedilum purpuratum have not been investigated. (3) The life forms of 173 protected plants were divided into 5 types, mainly phanerophytes ( 115 species ), followed by hemicryptophytes ( 34 species ) and hemicryptophytes ( 22 species ), and hemicryptophytes and cushion plants were the least ( 1 species ). (4) At the family level, Orchidaceae had the most species, including 47 species ( 27.17% ). At the genus level, Dendrobium has the largest number of species, including 21 species ( 12.14% ). (5) The floristic elements are complex and have obvious tropical characteristics. The families are mainly distributed in the pantropics and their variants. The genera are mainly distributed in tropical Asia and its variants. In summary, the tropical nature and unique phenomena of national key protected wild plants in Hainan Province are obvious, and there are many new protected species. Therefore, background investigation and dynamic monitoring should be carried out continuously, law enforcement should be strengthened, human factors should be reduced to interfere with and destroy habitats, and in-situ and ex-situ protection measures should be taken to strengthen protection and management.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》
Abstract： Vegetation restoration is an effective way for ecological restoration in the dry-hot valley, and many alien species are introduced along with natural vegetation restoration. In order to compare the responses of native and alien species to water and microorganisms, the native Albizia kalkora and alien Leucaena leucocephala were selected as our research objects, and the effects of water and soil microorganisms on the growth and development of the two plants were studied by soil moisture control and soil microbial inoculation under climate chamber conditions. The results were as follows: (1) Compared with Leucaena leucocephala, the seed germination rate, root biomass, root-shoot ratio and plant nitrogen-phosphorus ratio of Albizia kalkora were significantly increased by 58.82%, 76.13%, 90.59% and 18.42%, respectively (P<0.05); (2) Under the drought conditions, the root biomass and plant nitrogen concentration of Albizia kalkora were significantly lower than those under moist conditions by 33.67% and 8.65% (P<0.05), and the plant height, aboveground biomass and root biomass of Leucaena leucocephala under drought conditions were 39.32%, 40.06% and 39.61% lower than those under moist conditions (P<0.05), indicating that drought inhibited the growth and nutrient uptake of both plants; (3) The number of nodules in the roots of Albizia kalkora under drought conditions was higher than that under moist conditions (P<0.05), while the number of nodules in the roots of Leucaena leucocephala under moist conditions was higher than that under drought conditions; (4) Except for the proportion of dead leaf biomass, soil microbial inoculation and the interaction between soil microorganisms and water had no significant effect on the growth of Albizia kalkora and Leucaena leucocephala. The above results suggest that Albizia kalkora is more suitable for drought environment than Leucaena leucocephala, which provides a theoretical basis for the selection of species for vegetation restoration in dry-hot valleys, but the effects of soil microorganisms on plant growth are worthy of further study.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》
Abstract： Bsang is a folk activity with unique ethnic cultural characteristics on the Qinghai-Tibet Plateau (QTP), and it plays an important role in the daily life of Zang people. There are abundant bsang plant resources and related bsang cultural knowledge on the QTP, to investigate, document, and research Xizang bsang plants and related traditional knowledge, the research team conducted four ethnobotanical surveys on bsang plants in Xizang. Interviews were conducted with 459 informants from 22 townships, documenting 83 species of bsang plants belonging to 36 genera across 19 families. The results were as follows: (1) People of different regions, ages, and genders possess extensive knowledge of bsang plants; (2) Hordeum vulgare var. coeleste, Juniperus indica, Rhododendron anthopogon and have high cultural value (CV) indices, making them crucial in bsang activities; (3) The utilization of bsang plants demonstrates regional and substitutive characteristics, reflecting the local practices of scientifically managing and sustainably collecting of bsang plant resources. This study contributes to the inheritance of bsang activities and the biodiversity conservation and sustainable utilization of bsang plant resources.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-07 Cooperative journals: 《广西植物》
Abstract： L igularia virgaurea i s one of the original plants of the Tibetan medicine Rixiao for the trearment of clearing heat and removing yellow water. In order t o study the chemical constituents and anti inflammatory activity of L. virgaurea , the compounds were separated by silica gel , Sephadex LH-20 gel, ODS gel column chromatography and other column chromatography technologies. The structures of all isolates were identified by spectroscopic methods (NMR and HR ESI MS)MS). T he ir inhibitory activity of the compounds on nitric oxide (NO) w as determined by lipopolysaccharide (LPS) induced RAW26 4 7 cell model. The results were as follows: (1) Twenty one compounds were separated and identified from petroleum ether and n butanol extracts of L. virgaurea , including spiroeuryolide 1 )), cacalol ac etate 2 )), o plopenone 3 )), 8 ethyl palmosalide A 4 )), 1 hydroxy 3,7 dimethyl 2 --(pent 3 enyl)benzofuran 5 syringaresinol O β D gluco pyrano side 6 ), pinoresinol O β D gluco pyrano side 7 ), isoeucommin A 8 )), eucommin A 9 6,7 dimethoxycoumarin 10 )), fer ulic acid 11 ethyl caffeate 12 methyl caffeate 13 ), methyl ferul ate 14 ), ethyl ferulate 15 ), caffeic acid 16 ), 2 --[( E 3′,7′ d imethyl 2′,6′ octadienyl] 4 methoxy 6 methylphenol ( 1 7 2,8 dimethyl 6 methoxy 2 --(4 methylpent 3 enyl) chromene 1 8 β s itosterol 19 ), dodecyl(Z)-9-hexadecenoate (2020) and hexacosanal 21 Compounds 1 4 , 6 , 11 16 , 18 , 2 0 , 21 were isolated from the whole herbs of L virgaurea for the first time . (2) The anti inflammatory activity in vitro showed that compounds 1 3 6 11 16 , 17 19 could significantly inhibited releases of NO at concentration ranging from 1.56 to 50.00 μmol·L 1 P < 0.05 or P < C ompound 5 had no inhibitory release of NO at a concentration of 50.00 μmol·L 1 , but it could inhibit releases of NO a t concentration of 12.50 and 25.00 μmol·L 1 P < This finding enriches the chemical composition and biological activity research of L. virgaurea and provides a certain foundation for the future development and utilization of its anti inflammatory ac tivity.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-07 Cooperative journals: 《广西植物》
ZHANG Xiaohui
ZHANG Jianqiang
NING Kun
WANG Xiaojia
KANG Juqing
QIU Linfeng
LI Yali
LIU Xiaoying
YUE Ming
Abstract： Based on the references of natural reserves in Shaanxi P rovince field survey, and digitized herbarium specimens provided by China V irtual Herbarium (CVH), the resource and conservation status of species listed in national key protected wild plants ( (2021 version ) distributed in Shaanxi Province was investigated and analyzed in present study, and it can provide a scientific basis for future research on in situ and ex situ conservation . The results were as follows : (1) A total of 104 species (including subspecies and variety) were belonged to 38 families, 67 genera were distributed in Shaanxi province, including 3 species of Lycophytes, 6 species and 2 varieties of Gymnosperms, and 85 species, 1 subspecies and 10 varieties of Angiosperms, representing 2.88%, 6.73% and 90.38% of the total NKPWPs respectively. (2) The number of the NKPWPs represent ed about 18.18%, 5.09%, and 2% of families, genera and species respectively of the total vascular plant in Shaanxi P rovince . (3) Among 104 species of NKPWPs , 26 species listed in the last version (1999) ha d been protected , and 24 species were ever listed and protected as local protected wild plants , and a total of 54 additional species were not protected at all before, accounting for 52% of the total NKPWPs, e.g. Phalaenopsis zhejiangensis, Dendrobium flexicaule and Paeonia rockii. The endangered status analysis showed that 5 species are Critically Endangered (CR), representing 4.81%, 22 species were Endangered (EN) and 22 were Vulnerable (VU), representing 24.04% of the total NKPWPsNKPWPs. (4) Qinling-Bashan region was a concentrated distribution area of NKPWPsNKPWPs, about 60 species were distributed in Zhenping County, accounting for 57.69% of the total, and 47 species were distributed in both Pingli and Foping counties; contrast to it, few species found in the north area of Shaanxi Province. (5) There were 249 reserves in Shaanxi Province, including the natural reserve (national and provincial), wetland parks and forest parks, and about 70 species representing 67.31% of NKPWPs had been effectively protected in these areas. We suggest: (1) investigate the resources, distribution and status of the additional species of NKPWPs in Shaanxi Province Province, especially focus on the 54 species which are not previously protected; (2) facilitate the regional botanical gardens in the Loess Plateau area of Shaanxi Province; (3) dynamic monitor and update data of NKPWPs in natural reserves, so as to provide a scientific and valuable reference for in situ and ex situ conservation in the future.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-07 Cooperative journals: 《广西植物》
ZHANG Shunwei
LI Jinghan
YANG Xiaobo
LI Donghai
WANG Chongyang
ZHU Zicheng
JIANG Yuexin
HE Yiqi
Shang Naiyan
DU Chunyan
LIU Chang
ZHONG Iishuang
SU Xin
Abstract： In order to reveal the distribution and habitat characteristics of national key protected wild plants in Hainan Province, this paper selected national key protected wild plants, based on the 2021 edition of the List of national key protected wild plants th rough field surveys, researched the distribution of national key protected wild plants in Hainan Province and their type of vegetation. The results were as follows : (1) T here were a total of 53 families, 83 genera and 173 species of national key protected wild plants in Hainan Province, ranking fifth in the number of species and first in the density of species in China (based on the current data base of wild). (2) A ll 19 counties and municipalities in Hainan Province had national key protected wild plants, and the number of species in the central and the south of the cities/counties is much larger than that in the north, with the largest species richness in Changjiang Li Autonomous County (75 species), and the highest species density in Lingshui Li Autonomou s County (0.60 4 5 ind.•km 2 ); T he number of species of national key protected wild plants was distributed in nine gradients (with a gradient value of 200 m) from 0 to 1 867 m above sea level, and there were a trend of slightly decreased very rapidly increa sed slowly decreased, with the highest number of species distributed in the elevation band. When 800 m Altitude < 1 000 m and 1 000 m Altitude < 1 200 m, there was 81 national key protected wild plant species, while only 9 species in the segment 1 600 m Altitude < 1 867 m (3) The habitats of national key protected wild plants in Hainan Province were complex ed , rel ied on a total of 11 vegetation types, among which the lowland rainforest ha d the highest species richness (114 species), followed by mountain rainforest (105 species) and the semi mangrove forest (1 species); and the most severely externally disturbed is freshwater wet grassland, followed by lowland rainforest, and the least is m ontane cloud forest. The results of the study can provide scientific reference for the conservation and utilization of national key protected wild p lants in Hainan Province.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-07 Cooperative journals: 《广西植物》
Abstract： Rare and endangered wild plants are important s trategic resources and an essential component of biodiversity conservation. Grasping the diversity and geographic distribution of key protected plants in Jiangxi Province is a key step in developing scientific conservation and management at the provincial scale. Based on combined checklist of the Nat ional Key P rotected W ild P lants (NKPWP, 2021) and the checklist of the K ey P rotected W ild P lants in Jiangxi Province (KPWP, 2005), and digitized herbarium specimens provided by the National Specimen Information Infrastructure (NSII), the diversity, geograp hic distribution, and conservation profile of these plants found in Jiangxi P rovince are analyzed. T he results are as follows : (1) Total of 148 species of NKPWP (including intraspecies taxa), spanning 89 genera and 47 families, are found in Jiangxi provinc e . (2 ) Total of 407 species, belonging to 85 families and 208 genera, are recognized as the key protected plants in Jiangxi province, including 16 species of lycopods and ferns (9 families and 10 genera); 26 species of gymnosperms (6 families and 17 genera), and 365 species of angiosperms (70 families and 181 genera ). 3 ) Among of these species, 60.9 % of them are protected within 32 national and provincial natural reserves; 70.5 % of them are conserved ex situ in 67 Chinese botanic gardens . 4 ) T he hotspots of the key protected wild plants are identified, including mountains and its adjacent areas of Lushan , Jiuling, Wugong , Jinggang , Dayuling , Jiulian and Wuyi. The distribution pattern of hotspots is generally consistent with the range s of five key protected areas of biodiversity conservation in Jiangxi Province. The issues of the species included in the checklist of the key protected wild plants of Jiangxi Province (2005) were discussed, and the 6E principles of the priority selection of key protec ted wild plants at province level and the related suggestions to strengthen the research on provincial protected plants were proposed.
Subjects: Physics >> Nuclear Physics Subjects: Biology >> Radiobiology submitted time 2024-05-06
Abstract： Manned spaceflight and nuclear technology applications are running on a highway in China today. The radiation and nuclear safety will continue to be a major national demand in a long term. Thus, the continuous observation of new radiation protection molecular targets and related drugs is of great value to us. Our previous study has found that the circulating Insulin-like Growth Factor Binding Protein 3 (IGFBP-3) showed a significant increase after total body exposure of mice to ionizing radiation. However, the function of IGFBP-3 and the effects of it level change on radiation induced damages are still unclear. In this study, we set up the Igfbp3 gene overexpression and knock-down cell models in mouse Kupffer (MKC) cells. The CCK-8 assay, EdU assay, clone formation assay and microsphere phagocytosis experiment were performed for investing the proliferation activity, DNA replication activity and phagocytic ability of different cell models after carbon-ion irradiation. Moreover，mice were tail vein injected with recombinant IGFBP-3 protein at 2 hours before 5 Gy carbon-ion irradiation, and the survival curves of mice were drawn. The results showed that overexpression of IGFBP-3 protein significantly alleviated the radiation-induced decrease of the DNA replication activity, cell viability, clone formation rate, and phagocytic ability of MKC cells. On the contrary, the knock-down of IGFBP-3 protein expression reduced the above results. Injection of IGFBP-3 protein before carbon-ion exposure significantly delayed the time of death in mice. Our results indicate at the cellular and animal levels that IGFBP-3 protein has the potential to reduce radiation-induced damages and serve as a target for radiation protection. Through enhancing the radiation resistance and phagocytic ability of Kupffer cells in mice to reduce the risk of infection after radiation exposure might be the underlying mechanism of the effects of IGFBP-3 on radiation protection.
Subjects: Biology >> Biochemistry submitted time 2024-04-24
Abstract： How to differentiate the diagnosis of depression and bipolar disorder has always been an important problem that needs to be solved urgently in clinical practice. In this study, from the perspective of urine proteomics, urine samples of similar age were collected from two hospitals to investigate the candidate biomarkers for differentiating the diagnosis of depression and bipolar disorder using both group analysis and one-to-many analysis. The experimental results of the paired group analysis showed that 108 differential proteins were identified in the depressed group compared to the bipolar group under strict screening conditions with screening criteria of FC ≥ 2 or ≤ 0.5 and a two-tailed unpaired t-test of P < 0.01, with an average of 3.7 randomly generated differential proteins, and a confidence level of 96.6 % for the correlation between these proteins and the disease difference. In the one-to-many analysis, 24 differential proteins were co-identified by the samples of 13 depressed patients, 16 of which showed a completely consistent trend of expression changes in all depressed patients studied, and 6 of which were associated with immunoglobulins; 41 differential proteins were co-identified by the samples of 12 depressed patients out of 13, and 19 of which showed a completely consistent trend of expression change in the These results reflect the strong consistency of differential proteins between the two groups of patients. 12 or more samples from depressed patients were enriched for differential proteins related to multiple biological processes and signaling pathways associated with the immune system, which is consistent with previous studies: immune mechanisms may be one of the pathogenetic mechanisms of major depression and that drugs with major immune targets can improve depressive symptoms. In the future, it may be possible to observe the immune status of patients with depression to provide direction and basis for the precise treatment of depression. The results of this paper show that urine proteomics can differentiate between depression and bipolar disorder, suggest possible mechanisms and potential targets for the treatment of depression and bipolar disorder, and provide a tool for future differential diagnosis and precision treatment of the diseases.
Peer Review Status:Awaiting Review
Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： To probe into the impacts of organ cadmium accumulation on endophytic bacteria in Blumea balsamifera, the methods of high-throughput sequencing of 16S rRNA gene V3-V4 region and molecular ecological network analysis were employed to study the effects of Cd accumulation in roots, stems and leaves of B. balsamifera on the community characteristics of endophytic bacteria under different exogenous cadmium treatments (0 and 2.0 mg·kg-1). The results were as follows: (1) In comparison with the control group without exogenous cadmium addition (0 mg·kg- 1, Cd0), the treatment group with cadmium spiking in soils (2.0 mg·kg-1, Cd2) promoted plant growth and the cumulative Cd contents in root, stem and leaf, with the order of leaf (16.75 mg·kg- 1) > stem (11.99 mg·kg-1) > root (3.96 mg·kg-1). (2) Alpha diversity analysis showed that the endophytic bacteria richness (Sobs, Ace and Chao indices) and diversity (Shannon and Simpson indices) for organs were the highest in roots, followed by stems and leaves under Cd0 and Cd2 treatments. Additionally, the indices of richness and diversity of endophytic bacteria for each organ under Cd2 treatment were superior to those under Cd0. (3) At the phylum level, Proteobacteria, Actinobacteriota and Firmicutes were the dominant bacterial phyla in all the organs for both treatments; At the genus level, Delftia was the main bacterial genus with the relative abundance ranged from 53.0% to 92.7% and 57.1% to 89.2% in the plant organs of Cd0 and Cd2, respectively; Certain similarities existed among the endophytic bacterial community structures of roots, stems and leaves of B. balsamifera, and Cd2 increased the proportion of mutual endophytic bacterial genera in roots, stems and leaves and that of unique endophytic ones in each organ (except the root). (4) LDA Effect Size analysis showed that there existed the differences on endophytic bacterial genera residing in different organs within a group and also the same organ between groups. (5) Redundancy analysis (RDA) showed that the contents of rhizosphere soil Cd and plant organ Cd were significantly correlated with the composition of endophytic bacterial community. (6) Co-occurrence network analysis clarified that cadmium accumulation in B. balsamifera complicated the interaction network of endophytic bacteria occurring in root and leaf, and enhanced the competition among endophytic bacterial species in root and stem, and the symbiosis in leaf. In summary, exogenous Cd treatment affected the community structure and interaction mode of endophytic bacteria in B. balsamifera organs.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： Leaves are the largest and more sensitive vegetative organs exposed to external environmental conditions. In this paper, we aim to investigate the effects of arid habitat on the leaf anatomical structure of wetland plants, Carex moorcroftii leaf plots were set up along the gradient of arid habitat, and the response of leaf anatomical structure to arid habitat was investigated. The results were as follows: (1) The epidermal cells, vesicular cells and air cavity area on the distal surface of leaf tip and leaf bottom, leaf thickness and mechanical tissue thickness at leaf bottom were significantly positively correlated with soil volumetric moisture content (R2=0.06-0.34, P<0.01); The thickness of the cuticle, cell area, number of vascular bundles, and diameter of vascular bundles on the proximal axial surface of leaf apical, mesophyll, and basal regions were significantly negatively correlated with soil volumetric moisture content (R2=0.08-0.53, P<0.01). (2) The anatomical structure of leaf blade of C. moorcroftii had great plasticity (0.53-0.94) and variability (18%-63%), and vesicular cells, air cavities, and epidermal cells of the proximal axial surface had the greatest plasticity and variability, and the plasticity index and the coefficient of variation of the anatomical structure of the leaf bottom were significantly higher than that of the leaf tip and the middle part of the leaf (P<0.05). The plasticity of cuticle thickness and epidermal cell area on the proximal axial surface was significantly greater than that on the distal axial surface (P<0.05). When the habitat was aridified, C. moorcroftii leaves adapt to the arid habitat by thickening the cuticle on the proximal axial surface, increasing the epidermal cell area, decreasing the area of air cavities, and differentiating vesicular cells, and mainly adopted protective and frugal strategies to adapt to the arid habitat. The results of this study are helpful to reveal the response strategies of C. moorcroftii leaf anatomical structure to arid habitat, and provide theoretical reference for the protection and vegetation restoration of alpine meadow.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： Mangrove forests, characterized by high photosynthetic rates and low light compensation point, exhibit high Gross Primary Production (GPP), an important component of "blue carbon". Accurate estimation of regional GPP and quantification of its limiting factors are greatly significant for China to achieve its carbon neutrality goals. In this paper, we estimated the GPP of mangrove ecosystems in Hainan Island from 2016 to 2020 based on the Mangrove Vegetation Photosynthesis Light Use Efficiency (MVP-LUE) model using Sentinel-2 imagery and environmental data; and we also explored the spatiotemporal distribution pattern of GPP and its driving mechanism. The results were as follows: (1) During the study period, the annual GPP of mangrove ecosystems on Hainan Island showed an increasing trend, with higher GPP in the eastern than western regions and northern than southern regions. The mangroves distributed over a large area in northeastern Hainan Island dominate the temporal variation patterns at the whole island scale. However, distinct differences exist in the temporal dynamics across different regions of the mangrove ecosystem in Hainan Island. (2) In terms of the formation mechanism, the spatial and temporal distribution pattern of GPP of mangrove ecosystems on Hainan Island was driven by a combination of multiple meteorological factors. Seasonally within each year, during the transition from the dry season to rainy season, GPP was higher due to lower limitation from air temperature (Tair), and the promoting effects of photosynthetically active radiation (PAR) and sea surface temperature; in the middle of the dry season, low Tair imposed a serious limitation on GPP, but this Tair limitation was weakened with decreasing latitude. In the rainy season, higher cloud cover resulted in PAR becoming a limiting factor for GPP. At the end of the paper, we discussed the uncertainties and limitations of MVP-LUE, and the spatiotemporal distribution patterns of mangrove ecosystem GPP not only be constrained by environmental factors, but species composition and forest age structure can also be driving factors. The results of this study provide basic data to assess the contribution of regional mangrove forests to the global carbon cycle, and theoretical support to reveal the key environmental factors affecting mangrove ecosystem carbon dynamics.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： To explore the effect of exogenous polyamines (PAs) on embryogenic callus (EC) proliferation and somatic embryogenesis of litchi, the morphology, structure, endogenous PA content and related enzyme activities were systematically investigated using the ‘Feizixiao’ ECs as materials subcultured on the medium supplemented with various PAs. The results were as follows: (1) The exogenous putrescine (Put), spermidine (Spd), and spermine (Spm) treatment significantly increased the EC proliferation rate and reduced the amount of induced somatic embryos and regenerated plantlets. The proliferated embryogenic cells after exogenous PA treatments were more consistent in size and stained deeply and evenly. Furthermore, multicellular proembryos in EC were reduced, and fully differentiated early cotyledon embryos could be seen. (2) All the exogenous PA treatments significantly increased the endogenous PA content in EC. Among them, Put treatment had the highest content of each endogenous PA component and total PA. When the EC proliferated on the medium containing exogenous PAs was transferred to the medium without exogenous PAs (M3) for proliferating, the Put content in the EC was still significantly higher than the control, however, the endogenous Spd and Spm were significantly decreased. (3) Exogenous Put treatment significantly increased the activities of ornithine decarboxylase (ODC), arginine decarboxylase (ADC), and diamine oxidase (DAO) in EC, while exogenous Spd and Spm treatments significantly reduced the activities of ADC and DAO in EC, and exogenous Spd significantly increased PAO activity. When transferred to the M3 medium, the ADC and DAO activities of newly proliferated EC were significantly lower than those of EC cultured with exogenous PAs, but there was no significant difference in ODC and PAO activities. In summary, the exogenous PAs can affect endogenous PA content by regulating the activity of enzymes related to polyamine metabolism, thereby affecting EC proliferation and somatic embryo induction in litchi. These results would provide a basis for further study on the mechanism of PAs regulating litchi regeneration in vitro.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-03-26 Cooperative journals: 《广西植物》
Abstract： Rosa praelucens is a critically endangered alpine wild flower endemic to Shangrila County of Yunnan Province. Rich in phenotypic diversity and with a high ploidy level of decaploid, R. praelucens is a very important rose germplasm resource. In order to clarify the genetic background of its phenotypic variation, the chloroplast genomes of 40 individual plants representing different phenotypes within the species were sequenced by using the Illumina HiSeq 2000 platform, and then assembled, annotated and compared. The results were as follows: (1) Chloroplast genomes of R. praelucens were 157 173-157 261 bp in length, with a size difference of 88 bp among different individual plants. The genomes encoded 132 genes, mainly related with photosynthesis and self-replication. 27 155 codons, preferring using codon ending of A or U, were found in all the coding sequences. (2) Thirty six repeats and 73 simple sequence repeats (SSRs) were detected in the chloroplast genome of R. praelucens. Most of the cpSSRs were mononucleotide type and located in the intergenic region of LSC region. (3) The haplotype diversity (Hd) among the 40 chloroplast genomes was 0.928±0.027, and the nucleotide diversity (Pi）was 0.00012. The intergenic region of petN-trnD and psaA-ycf3, gene rps16 and ycf1 were relatively more divergent. No reverse or loss of large DNA fragments and genes were found among the cp genomes of different individuals. These results suggested that the chloroplast genomes were highly conserved in size, sequence and structure within R. praelucens. The rich intraspecific phenotypic diversity was not directly caused by the variation of chloroplast genomes among different individual plants.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-03-26 Cooperative journals: 《广西植物》
Abstract： In order to investigate the response mechanism of Ranunculus japonicus, Thalictrum fortunei and Delphinium anthriscifolium var. savatieri in different light environments, five shade treatment levels (0%, 30%, 50%, 70% and 90% shade degree) were set in this study, the photosynthetic indexes of the three plants were determined, and the effects of different shade treatments on their photosynthetic characteristics and shade-tolerance evaluation were studied. The results were as follows: with the increase of shading degree, (1) The contents of chlorophyll a, chlorophyll b, chlorophyll (a+b) and carotenoid increased, while the content of chlorophyll a/b decreased with further increase of the shade degree of stress. (2) The apparent quantum efficiency (AQY) of the three plants increased first and then decreased;The maximum net photosynthetic rate (Pmax) of R. japonicus and D. anthriscifolium var. savatieri showed a trend of first rising and then decreasing, while the Pmax of T. fortunei showed a decreasing trend.The light saturation point (LSP), light compensation point (LCP) and dark respiration rate(Rd) of the three plants decreased gradually. (3) The original fluorescence (Fo) decreased first and then increased, while the maximum fluorescence (Fm), variable fluorescence (Fv), maximum photochemical efficiency of PSⅡ (Fv/Fm) and potential activity of PSⅡ (Fv/Fo) values increased first and then decreased; The quantum ratio of heat dissipation (φDo) and the energy dissipated per unit reaction center (DIo/RC) decreased first and then increased, while electron transport quantum yield(φEo), light energy absorbed per unit reaction center (ABS/RC), light energy captured per unit reaction center (TRo/RC), energy used to transfer electrons per unit reaction center (ETo/RC), photosynthetic performance index (PIabs) and comprehensive performance index (PItotal) increased first and then decreased. (4) Through comprehensive analysis of 20 single indicators by using analysis methods such as principal component analysis and membership function method. The shade-tolerance of the three plants ranked as T. fortunei > D. anthriscifolium var. savatieri > R. japonicus. In conclusion, the three species of goldenseal have different adaptability to light, which provides a basis for the application of goldenseal in landscaping.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-03-26 Cooperative journals: 《广西植物》
Abstract： To promote the restoration and reconstruction of ecosystem in the water-land ecotone, based on typical sample investigation, the variation patterns of vegetation species composition and diversity, and soil nutrients under different vegetation types were studied using Pearson correlation coefficient method and redundancy analysis method. The results were as follows: (1) There were significant differences in plant community structure and species diversity of different vegetation types (gravel zone, grass zone, shrub-grass zone, trees zone). As the submersed duration decreased, the water-land ecotone gradually evolved from scattered herbaceous plant communities to grass, shrub, and tree plant communities, and the α diversity of vegetation species (Shannon-Wiener index, Pielou index and Simpson index ) and vegetation coverage showed a gradually increasing trend, which were lowest value on the gravel zone and the highest on the trees zone. (2) There were significant differences in soil nutrient content among different vegetation types. With the decreased of submersed duration, soil organic matter content gradually increased, while soil water content, available nitrogen, available phosphorus, and available potassium showed a trend of first increasing and then decreasing, the maximum values of these nutrients mostly occurred in shrub-grass zone or trees zone, followed by grass zone, and gravel zone was the lowest. (3) Correlation and redundancy analysis showed that soil available nitrogen, available potassium, available phosphorus and organic matter were significantly positively correlated with various indicators of vegetation species α diversity, among which soil available nitrogen and available potassium had the strongest correlation with vegetation species diversity. In conclusion: different vegetation types in the Lijiang water-land ecotone have heterogeneous patterns of vegetation species composition and diversity as well as soil nutrients. Moderate submergence is beneficial for vegetation community aggregation and promoting soil nutrient accumulation. Herbaceous plants have stronger adaptability to moderate submergence environments. During the ecological restoration process of the Lijiang water-land ecotone, it is necessary to design restoration plans for different vegetation types and fully consider the relationship between vegetation species diversity and soil available nutrients.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-02-07 Cooperative journals: 《广西植物》
Abstract： In order to investigate study the influence of microbial agent Bacillus natto on the ability of Dicranopteris pedata to tolerate high temperature stress, this study determined the physiological indexes of photosynthesis and resistance to high temperature of annual D. pedata under different temperatures and fertilizers treatments. The results showed that: (1) both temperature and fertilizer could significantly affect the photosynthesis and high temperature resistance physiology of D. pedata (P＜0.05). (2) The net photosynthetic rate, transpiration rate, stomatal conductance, and chlorophyll content of D. pedata leaves were significantly reduced (P ＜0.05) when the temperature was increased to 45℃. The inhibition of photosynthetic physiology of D. pedata by high-temperature stress was an non-stomatal limiting. To defend the high temperature stress, the superoxide dismutase, peroxidase activity, proline, malondialdehyde content, and relative conductivity of D. pedata were increased significantly (P ＜ 0.05). (3) Compared with the control group and the organic fertilizer group, the fertilizer with microbial agent audition significantly increased the net photosynthetic rate, transpiration rate, stomatal conductance, chlorophyll content, and the activities of peroxide dismutase and catalase in leaves of D. pedata, and decreased the intercellular CO2 concentration, malondialdehyde content and the relative electrical conductivity of D. pedata leaves (P < 0.05). (4) The evaluation method combining principal component analysis and membership function method was used to evaluate the high temperature resistance of D. pedata. It was found that the D. pedata scored higher in resistance to high temperature under fertilizer-added origin manure application. Overall, D. pedata has a certain degree of resistance to high temperature, and can selectively induce stress resistance physiology to adapt the high temperatures environment according to different stress environments. The Bacillus natto can alleviate the photosynthesis inhibition of D. pedata by high temperature stress, induce the increase of antioxidant enzyme activities to alleviate cell damage, reduce the pressure of osmoregulation, and effectively improved the resistance of D. pedata induced by high temperature stress. This study provide a reference for the ecological restoration and soil and water conservation in the southern red soil erosion area and similar areas.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-02-07 Cooperative journals: 《广西植物》
Abstract： Investigating the variation patterns of hydraulic traits of trees with different diameters at breast height (DBH) grades has important physiological significance for understanding the water transport mechanism in plants. We focused a dominant tree species (Lannea coromandelica) in the dry and hot ecosystem of the Yuanjiang River, and measured the distribution patterns of hydraulic traits including shoot- (leaves and stems), leaf-, and stem hydraulic conductance, and morphological properties (diameter at breast height, leaf area/petiole length, leaf area/mass of petiole, mass of leaf/mass of petiole, average petiole length, bark density, wood density, Huber value and leaf mass per area) for 56 L. coromandelica individuals with different DBH (0 < DBH ≤ 9 cm), and then discussed the variation patterns of these traits among these three DBH levels (0 < DBH ≤ 3, 3 < DBH ≤ 6 and 6 < DBH ≤ 9 cm). The results were as follows: (1) the stem hydraulic conductance was significantly greater than leaf hydraulic conductance in three DBH grades, indicating that there was a hydraulic segmentation between the stem and leaves, whereas the degree of hydraulic segmentation had a similar pattern. (2) Among the three different diameter grades, there were significant differences in morphological traits such as average petiole length, bark density, wood density, Huber value and leaf mass per area, but there were no significant differences in the hydraulic conductance of the shoot, stem, and leaf. (3) Among the three diameter grades, the shoot hydraulic conductance was positively correlated with leaf and stem hydraulic conductance, indicating there was a coordination between leaf and stem hydraulic conductance. The shoot hydraulic conductance was negatively correlated with bark density and wood density, and leaf hydraulic conductance was significantly negatively correlated with the Huber value, indicating these leaf and stem morphological properties may impact shoot and leaf hydraulic conductance. Overall, this study suggest that there are similar hydraulic distribution patterns between leaves and the stem of L. coromandelica at different growth stages, although some morphological traits differ significantly among different diameter grades. These results improve our understanding for the intra-specific variation in leaf and stem hydraulic traits of valley savanna plants.
Hits
Hits
Downloads
Comment
YES