Physiological response characteristics of Hippophae rhamnoides seedlings under interaction of Mn stress and sex-specific competition

Subjects: Biology >> Botany >> Applied botany submitted time 2022-08-05 Cooperative journals: 《广西植物》

方玲 林玉虎 何云晓 陈浩 徐元洪 孙旭东 陈娟

Abstract： Sea buckthorn (Hippophae rhamnoides) is an important dioecious tree species of protective plantation with insufficiently documented on the sexual responses difference to environmental stress as well as the interaction between sexual competition and heavy metal stress. In order to provide the practical guidance for repairing heavy metal pollution in soil, the physiological responses and tolerance of male and female sea buckthorn plants under Mn stress and different gender interaction patterns were discussed. The physiological responses of sea buckthorn under Mn stress ( 4 000 mg·kg-1 ) and three different gender combinations ( female× female, male×male, female × male) were determined, including the contents of chlorophyll, peroxidase (POD), superoxide dismutase (SOD), malondialdehyde (MDA), total phenols, free proline (Pro), soluble sugar (SS), betaine and manganese in male and female leaves. The results were as follows： (1) Under Mn stress, the SOD of male plants (M/FM) was the highest in all competition combinations, while the MDA content was not significantly increased compared with the controls, indicating that the male plants had better antioxidant capacity and less membrane oxidative damage. (2) M/FM accumulated more proline and showed better osmotic adjustment ability under Mn stress, indicating that male plants under sexual competition showed better tolerance to Mn stress. (3) The study indicated that male-female interaction and Mn stress interaction significantly affected the physiological response characteristics of sea buckthorn leaves, such as chlorophyll content, antioxidant enzyme activity and osmotic adjustment. Principal component analysis showed that the contents of SOD, POD, MDA, chlorophyll b (Chlb), SS and Pro in leaves could be used as the main physiological response indicators. The results can be used as a reference for the remediation of soil heavy metal pollution by sea buckthorn plants.

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