Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： The pharmacognostical study of Bauhinia aurea was conducted, and identification methods and quantitative determination method were established to solve the problem of its authenticity, analyze the reasons for its different clinical efficacy. The methods of primary identification, morphological identification, microscopic identification, TLC identification and HPLC were used in the pharmacognostical study of B. aurea. The results were as follows: (1) It is a perennial stout woody vine with reddish brown hairs on the surface of stems, leaves, flowers and fruits. (2) A large number of stone cell groups and crystal fibers could be seen in the cortex of the transverse section of stems. The calcium oxalate cluster crystals and square crystals were scattered in the parenchyma of pith. The cross section of the leaves showed that the leaves were bifacial leaves. Calcium oxalate cluster crystals and square crystals were commonly found in the cortex. Secretory cavities were arranged intermittently into a ring in the phloem. Crystal fibers were often seen around the phloem. Calcium oxalate cluster crystals, crystal fibers, stone cells, bordered pit vessels, non-glandular hairs and stomatas could be found in the powder. (3) The spots of samples were displayed in the same color as the reference substances in the same position of the TLC figure. (4) The linear ranges of astilbin and quercitin were 0.005 888~2.355 μg (R=0.999 6) and 0.039 55-1.582 μg (R=0.999 8). The average recoveries (n=6) were 96.42% (RSD=2.55%) and 104.2% (RSD=1.79%). Pharmacognostical identification methods and a HPLC method for simultaneous content determination of two components astilbin and quercitin in B. aurea were developed. The methods are simple, stable, accurate, which can be used as the basis to set up its quality standard.
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