Effects of nitrogen, phosphorus and potassium fertilizers on physiology and chlorogenic acid synthesis and accumulation of Pyrrosia petiolosa

Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》

BU Yuanyuan PAN Zhifang CUI Xiangxiang ZHOU Xiao CHEN Jianhua HUANG Rongshao LI Liangbo

Abstract： To explore the effects of nitrogen, phosphorus and potassium on the physiology of Pyrrosia petiolosa as well as the synthesis and accumulation of chlorogenic acid, tissue culture seedlings of P. petiolosa were used as materials, and three concentration gradients of low nutrient ( no fertilization : N0, P0, K0 ), normal fertilization ( N : 0.2 g·kg-1, P : 0.15g·kg-1, K : 0.15 g·kg-1 ) and high nutrient ( N1 : 0.4 g·kg-1, P1 : 0.3 g·kg-1, K1 : 0.3 g·kg-1) were set up. There were seven treatments set up: NPK、N0PK、N1PK、NP0K、NP1K、NPK0 and NPK1, and the resistance physiological indexes, chlorogenic acid content and key enzyme activities of P. petiolosa under different treatments were determined. The results were as follows: (1) Nitrogen, phosphorus and potassium fertilizers had a significant effect on the resistance physiology of P. petiolosa. The activity of superoxide dismutase(SOD) increased significantly under high nitrogen and low potassium treatments, but catalase (CAT) activity rose significantly under low and high treatments of the three nutrients. (2) The content of chlorogenic acid in P. petiolosa was significantly affected by different levels of nitrogen, phosphorus, and potassium. The chlorogenic acid level in normal fertilization was the highest, reaching 12.92 mg·g-1, while the chlorogenic acid content in high potassium fertilization was the lowest, 7.79 mg·g-1. Potassium fertilizer had the most significant effect on chlorogenic acid content. The content of chlorogenic acid was positively correlated with the activities of quinate o-hydroxycinnamoyl transferase (HQT) and coumaroyl coenzyme ligase (4CL), and negatively correlated with the activity of shikimate o-hydroxycinnamoyl transferase (HCT). HQT, 4CL and HCT were the key factors leading to the difference of chlorogenic acid content. The results of this study can provide a theoretical basis for the artificial cultivation of P. petiolosa.

 Hits 591  Downloads 94  Comment