Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： Saccharum officinarum is the main cash crops in sugar processing industry, and its leaves are characteristic Yao medicine in Guangxi Zhuang Autonomous Region with a long history. Our recent study showed that its EtOAc extract was the active part responsible for its in vitro antitumor activity. In order to clarify the chemical compositions of this part and provides some basis for it’s further development , the modern separation and purification techniques, such as silica gel column chromatography, Sephadex LH-20 column chromatography, and semi-preparative highperformance liquid chromatography were used to identify the structures of the isolates by their physical-chemical properties and modern spectral analysis. As a result, twenty compounds were isolated and identified as 3, 4-dihydroxybenzaldehyde (1), methyl 3, 4-dihydroxybenzoate(2), 3, 4-dihydroxy-benzoic acid (3), 3-hydroxy-4-methoxybenzoic acid (4),p-hydroxybenzoic acid (5), 4-hydroxybenzyl aldehyde (6), p-hydroxy-cinnamicacid (7), syringic acid (8), 3, 5dihydroxyhydroquinone (9),1-hydroxybenzoyl-4-O-α-L-rhamnopyranoside(10), phydroxybenzoyl- β-D-glucopyranoside (11), quercetin (12), tricin (13), tamarixetin (14),isorhamnetin (15), 5, 3', 4'-trihydroxy-7-methoxyflavanone (16), sternbin (17), ((E)4- (1S,3R,4R)-1-hydroxy-4,5,5-trimethyl-7-oxabicyclo[4.1.0]heptan-1-yl)but-1-en-3-o-ne(18), blumenol A (19) and thymidine (20), respectively. Compounds 1-4, 6, 9-11, 13-16, 18 and 20 were isolated and identified from this plant for the first time.
Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》
Abstract：无瓣海桑果实为真红树无瓣海桑的果实。研究无瓣海桑果实不同提取物对 D-半乳糖所致衰老小鼠学习记忆能力影响及其作用机制。采用 Morris 水迷宫实验测量无瓣海桑果实不同提取物对小鼠的学习记忆能力影响，HE 染色观察各组小鼠脑部神经细胞的变化情况，并测定脑组织超氧化物歧化酶（SOD）活力、谷胱甘肽过氧化物酶（GSH-Px）活力、一氧化氮（NO）含量和单胺氧化酶（MAO）活力。结果表明与模型组相比，无瓣海桑果实不同提取物处理组小鼠在水迷宫实验中逃避潜伏期明显缩短（P＜0.05），目标象限停留时间明显增加（P＜0.05）。无瓣海桑果实不同提取物处理组小鼠脑部神经细胞损伤与模型组相比明显减少，小鼠脑部 SOD 酶活力和 GSH-Px 酶活力提高（P＜0.05），NO 含量和 MAO 活力在脑部显著降低（P＜0.05）。无瓣海桑果实不同提取物对 D-半乳糖致衰老小鼠学习记忆能力有改善作用，无瓣海桑果实不同提取物通过提高小鼠脑内源抗氧化酶（SOD、GSH-Px）活力，降低脑部 NO 含量和 MAO 活力来提高 D-半乳糖致衰老小鼠的学习记忆能力。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-09-07 Cooperative journals: 《广西植物》
Abstract： The 95% ethanol extracts of the leaves of Rhizophora stylosa, stems and leaves of Kandelia candel, leaves of Pongamia pinnata and stems of Aegiceras corniculatum were extracted by ultrasonic extraction method. The cytotoxicity of 95% ethanol extracts from these 4 mangrove plants on prostate cancer cells DU145 and PC3 was tested by MTS assay. The results showed that the anti-proliferation on prostate cancer cell DU145 of 95% ethanol extract of A. corniculatum stems was the strongest among the tested samples with IC50 value of 75.23, 88.81 and 76.53 μg·mL-1 to DU145, respectively. On this basis, the ethanol extract of A. corniculatum stems was successively treated with petroleum ether, ethyl acetate and n-butanol to yield 4 fractions , then the ethyl acetate fraction of A. corniculatum stems (EACS) was tested for anticancer activities against 19 tumor cells (HT-29, SW480, DLD-1, COLO205, PC3, DU145, SKOV3-S, A2780, SGC-7901, Tca-8113, MDA-MB-231, HepG2, SMMC-7721, Bel-7402, MHCC-97H, Hela, PANC-1, EJ, A549) and antiproliferative effects of 3 normal cells (HUVEC, EC304, RWPE-1) by MTS assay, the effect of EACS on proliferation of HT-29, DLD-1, SW480, DU145, PC3 and SKOV3-S cells was detected by the colony formation assay. The results showed that EACS exhibited different degrees of inhibitory effect on proliferation of 16 tumor cells and 3 normal cells, and had the stronger inhibitory effect on the proliferation of RWPE-1 with IC50 value of 22.78 μg·mL-1 after 72 hours of drug treatment . EACS inhibited the colony formation of HT-29, DLD-1, SW480, DU145, PC3 and SKOV3-S cells in a dose-dependent manner. The results provided a scientific reference for the further development and utilization of the above 4 mangrove plants. At the same time, it was initially determined that A. corniculatum has significant anti-tumor activity and is expected to be further developed as a new anti-tumor drug.
Hits
Hits
Downloads
Comment