• Pollen morphology and leaf epidermal micromorphology of 10 species(Camellia, sect. Tuberculata)

    Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》

    Abstract: To provide researh data for the phylogenetic evolution, classification and identification of plants in sect. Tuberculata of Camellia genus, 10 species of the section, leaf epidermal micromorphology and pollen morphology were observed by light microscope and scanning electron microscopy (SEM) and based on pollen features and leaf epidermal features to clustered separately. The results were as follows: (1) 10 species have no significant difference in morphology and size, the pollen shape is prolate, subspheroidal or oblatesphere; the outline of pollen in polar view is trilete rounded or trilete regular teiangular; the outline in equatorial view is oblong, P/E rangs from 0.85 to 1.16; tricolporate aperature. The exine sculpture is different significantly, the featuee have coarsely warty, foveolate reticulate or rugulate with beadedmun and is important classification value for sect. Tuberculata. (2) The cluster analysis of the measurement index showed that when the euclidean distance is 4.5, the 10 species are divided into 4 groups, and some of the classification results are the same as the macroscopic morphological classification. (3) The leaf epidermal cells of 10 species are irregular or straight, with great morphological differences among species. There are gland in the upper and lower epidermis of C. zengii, C. rhytidophylla and C. rubimuricata; cyclic type stomata are only distributed in the lower epidermis; three shape of anticlinal wall pattern shape have sinuolate, sinuous, repand and sinuate. In addition, the cell size and stomatal features (size and density) of leaf epidermis micromorphology were significantly different between species. This study shows that pollen morphology and leaf epidermal micromorphology are diverse in the sect. Tuberculata, which could be used as the basis for distinguishing some closely related species in the sect. Tuberculata.