Subjects: Biology >> Botany >> Applied botany submitted time 2021-07-20 Cooperative journals: 《广西植物》
Abstract： To identify the molecular mechanisms of on sugarcane (Saccharum officinarum ) responding to cold stress, and find the miRNAs and target genes related to cold tolerance，the leaves of different sugarcane genotypes with different cold tolerance treated for 24 h at 4 ℃ were sampled as the materials for high-through transcriptome sequencing with IlluminaHiSeqTM 2000, and 18 sRNA libraries before and after cold stress were constructed. The results were as follows:（ 1）Total 322 known miRNAs of 84 families were discovered, 110 new miRNAs were predicted. 100 differentially expressed miRNAs were screened out from the known miRNAs (61 up-regulated, 39 down-regulated) and 37 differentially expressed miRNAs (15 up-regulated, 22 down-regulated) were screened out from the new miRNAs. （2）1 844 target genes were predicted by using psRNATarget, TargetFinder and Tapirhybrid software. Three main functional categories of these target genes were revealed via the functional analysis of gene ontology, namely molecular function, cellular component and biological process. （3）In order to verify the high-throughput sequencing data, 14 miRNAs and their target genes were selected for qRT-PCR analysis, which showed that most of the expressions of the 14 miRNAs were detected and consistent with the sequencing results. （4）Some miRNA target genes were identified, which involved in plant growth, development, and cold stress responses. This study shows that miRNA directly or indirectly regulates the expression of target genes in cold-tolerant sugarcane, and plays a key role in regulating the important agronomic traits.
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