Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-26 Cooperative journals: 《广西植物》
Abstract： In order to detect the effect of fertilization and mowing on stoichiometry characteristics of four plant species in an semi-arid steppe in Inner-Mongolia. Four plant species including Artemisia frigida, Potentilla tanacetifolia, Leymus chinensis, and Melissitus ruthenica in the steppe were collected in August 2014. Contents of carbon, nitrogen and phosphorus, and ratios of C:N, C:P and N:P in different plants in field experiment under nitrogen fertilization and mowing treatment were investigated. The effect of nitrogen fertilization and mowing on carbon, nitrogen and phosphorus, and ratios of C:N, C:P and N:P were analyzed with ANOVAs. The differences of stoichiometry characteristics under different treatments were analyzed with Post-Hoc tests. The results indicate that neither nitrogen fertilization nor mowing alter carbon content in the steppe. Nitrogen fertilization significantly elevated the plant nitrogen content (P < 0.05), thus decreased the C:N ratio (P < 0.05) and increased the N:P ratio (P < 0.05). Mowing increased the nitrogen content of M. ruthenica by 0.18% (P < 0.05), decreased the nitrogen content of L. chinensis by 0.13% (P < 0.05), but did not affect the nitrogen content of A. frigida or P. tanacetifolia. Mowing significantly elevated the phosphorus content of Artemisia frigida and Potentilla tanacetifolia (P < 0.05), but did not affect the phosphorus content of M. ruthenica or L. chinensis. The study has approved that nitrogen fertilization and mowing can affect the plant stoichiometry characteristics in the steppe, but the affect varied with species. The results can provide data support for the study on plant functional traits in the grassland.
Subjects: Biology >> Zoology submitted time 2017-10-10 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究陕北白绒山羊羯羊能量和蛋白质的需要量，为制定其饲养标准提供数据。选取36只体重相近、健康状况良好的陕北白绒山羊周岁羯羊，按3×3(能量×蛋白质)完全随机设计分为9组，每组4个重复，每个重复1只。各组饲粮消化能和粗蛋白质水平分别参照NRC（1981）推荐量的100%、110%、120%和90%、110%、130%设定。预试期7 d，正试期46 d。结果表明：1）饲粮能量水平对周岁羯羊的干物质采食量和平均日增重都有极显著影响(P<0.01)，蛋白质水平对干物质采食量影响显著(P<0.05)；饲粮能量和蛋白质水平对干物质采食量有显著的互作效应（P<0.05）。2）低能量水平组的可消化氮显著低于中能量水平组与高能量水平组(P<0.05)；低蛋白质水平组的进食氮、可消化氮、沉积氮、氮表观消化率均极显著低于中蛋白质水平组和高蛋白质水平组(P<0.01)。3）总能消化率和总能代谢率均随着能量水平的提高而极显著地升高（P<0.01）；高蛋白质水平组的总能代谢率和消化能代谢率显著低于低蛋白质水平组（P<0.05）；能量和蛋白质水平对总能消化率、总能代谢率和消化能代谢率均产生显著或极显著的互作效应（P<0.05或P<0.01）。本试验得到了陕北白绒山羊周岁羯羊能量和蛋白质需要量的回归方程，饲粮的消化能和代谢能分别以8.80～10.61 MJ/kg和7.34～8.76 MJ/kg较为适宜，粗蛋白质和可消化粗蛋白质分别以10.00%和7.34%为宜。
Subjects: Biology >> Bioengineering submitted time 2017-09-20
Abstract： In this paper, the strain ZZ-46 which could assilimated glucose and xylose simultaneously producing lipid were screened from 10 wild yeast strains from the soil of Nanyang region of henan province. The cell biomass, lipid concentration, lipid content, lipid coefficient and lipid productivty of the strain reached 20.23g/L、9.89 g/L、48.91%、14.64g/100g and 0.083 g•L-1•h-1 respectively by fermenting 120h with 2: 1of glucose and xylose as carbon source. Simultaneous, Lipid-acid component was analyzed, there is almost no difference in lipid-acids component by using different mixed sugar ratios as carbon source and C16 and C18 series are main fatty acids. Oleic acid content is the highest in lipid-acid component, next are linoleic acid, palmitic acid and stearic acid in all samples and the sum account for over 90%. The lipid component of this strain is similar to plant oil and could be used as biodiesel source. The strain was primarily identified as Cutaneotrichosporon dermatis by the morphology feature and 26S rDNA D1/D2 domain sequence analysis, and Trichosporon dermatis homonym.
Subjects: Biology >> Bioengineering submitted time 2017-06-01 Cooperative journals: 《中国生物工程杂志》
Abstract： Objective To investigate the mechanism underlying miR146a participating in the cardiotoxicity of doxorubicin. Methods Rat cardiomyocytes H9c2 were treated with doxorubicin (DOX). After that, the cell viability was detected by CCK-8, the miR146a variation was quantified by qPCR and the change of cleaved Caspase 3 was measured by Western blot (WB). Using CRISPR, two sgRNAs were designed to knockout (KO) the expression of miR146a. The cell viability and cleaved Caspase 3 of miR146a KO cells were respectively detected by CCK-8 and WB. The potential target of miR146a was predicted and validated by luciferase system. The change of target gene after DOX treatment, and the influence of miR146a KO on target gene were detected using WB. Results After DOX treatment, cell viability decreased, the level of cleaved Caspase 3 elevated and miR146a expression increased 3.6 folds. Using CRISPR, miR146a expression was successfully suppressed, with a much higher inhibitory rate than microRNA decoy (88.6% vs 57.6%). miR146a KO significant impeded DOX induced miR146a elevation, cell viability decrement and cleaved Caspase 3 increment. Smad family member 4（Smad4） was proved to be a target of miR146a by informatics prediction and luciferase experiment. The treatment of cardiomyocytes by DOX led to a decrease of Smad4 expression. In miR146a KO cells, the expression of Smad4 increased, which was not changed by DOX treatment. Conclusions In rat cardiomyocytes, miR146a participated in doxorubicin cardiotoxicity through Smad4.
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