南高丛蓝莓‘奥尼尔’工厂化组培快繁技术研究

Abstract: In order to establish a rapid propagation system of southern highbush blueberry ‘O’ Neal’ , the stem section with axillary bud were sterilized as explants, induction of shoot cluster,and proliferation of shoot cluster, test-tube rooting, transplanting and domestication were also studied. The results were as follows: The best sterilization treatment was that the explant treated by 10% NaClO with 15 min, and the contamination rate reached 14.44% and the induction rate reached 49.38%; The best medium for induction of shoot cluster was WPM+ ZT 1.5 mg‧L-1. The optimum medium for proliferation of shoot cluster was WPM+ NAA 0.1 mg‧L-1 + ZT 1.5 mg‧L-1, and the coefficient of propagation reached 8.6 after subcultured 60 days. The best treatment for test-tube rooting was that the shoot cluster dipped in 500 mg‧L-1 IBA for 20 s firstly, then cultivated them with the medium WPM+IBA 0.2 mg‧L-1, the rooting rate and effective rooting rate all reached 96.3%, and the rooting number and length were 12.7 and 43.3 mm after cultured 90 days, at the same time, and the root developed well. The survival rate of rooted seedlings reached 92.22% after transplanted in Media Ⅰ（river sand﹕vermiculite﹕perlite=1﹕1﹕1）. Thus, the best rapid propagation system of southern highbush blueberry ‘O’Neal’ was that: the explant treated by 10% NaClO with 15 min, the shoot cluster inducted and proliferated with WPM+ ZT 1.5 mg‧L-1 and WPM+ NAA 0.1 mg‧L-1 + ZT 1.5 mg‧L-1differently; The shoot cluster dipped in 500 mg‧L-1 IBA for 20 s and cultivated with WPM+ IBA 0.2 mg‧L-1, and rooted seedlings transplanted in media（river sand: vermiculite: perlite=1﹕1﹕1）. The results play a foundation for providing seedlings and factory production of ‘O’Neal’ in the future.