Current Location:home > Detailed Browse

Article Detail

SARS-coronavirus-2 nsp13 possesses NTPase and RNA helicase activities

Abstracts

The ongoing outbreak of Coronavirus Disease 2019 (COVID-19) has become a global pandemic and crisis. SARS-coronavirus-2 (SARS-CoV-2), the causative pathogen of COVID-19, is a positive-sense single-stranded RNA virus belonging to the family Coronaviridae. For RNA viruses, virus-encoded RNA helicases have long been recognized to play pivotal roles during viral life cycles by facilitating the correct folding and replication of viral RNAs. Here, our studies show that SARS-CoV-2-encoded nonstructural protein 13 (nsp13) possesses the nucleoside triphosphate hydrolase (NTPase) and RNA helicase activities that can hydrolyze all types of NTPs and unwind RNA helices dependently of the presence of NTP, and further characterize the biochemical characteristics of these two enzymatic activities associated with SARS-CoV-2 nsp13. Moreover, we found that some bismuth salts could effectively inhibit both the NTPase and RNA helicase activities of SARS-CoV-2 nsp13 in a dose-dependent manner. Thus, our findings demonstrate the NTPase and helicase activities of SARS-CoV-2 nsp13, which may play an important role in SARS-CoV-2 replication and serve as a target for antivirals.
Download Comment Hits:15746 Downloads:1619
From: 周溪
DOI:10.12074/202002.00081
Recommended references: Shu, Ting,Huang, Muhan,Wu, Di,Ren, Yujie,Zhang, Xueyi,Han, Yang,Mu, Jingfang,Wang, Ruibing,Qiu, Yang,Zhang, Ding-Yu,Zhou, Xi.(2020).SARS-coronavirus-2 nsp13 possesses NTPase and RNA helicase activities.[ChinaXiv:202002.00081] (Click&Copy)
Version History
[V2] 2020-04-19 16:43:00 chinaXiv:202002.00081V2 Download
[V1] 2020-02-27 15:51:18 chinaXiv:202002.00081v1(View This Version) Download
Related Paper

Download

Current Browse

Change Subject Browse

Cross Subject Browse

  • - NO