Subjects: Biology >> Botany submitted time 2024-06-02 Cooperative journals: 《广西植物》
Abstract： To investigate the characteristics of the fungal community and potential key pathogens, ITS rDNA gene amplicon sequencing technology and FUNGuild analysis were used to study the fungal community composition, network characteristics and ecological function groups in rotting tubers of Dioscorea. sp. Additionally, potential pathogenic fungi were isolated and identified. The results were as follows: ( 1 ) In rotting tubers of Dioscorea sp., the dominant phylum was Ascomycota, and prevalent fungal genera included Penicillium, Colletotrichum, Fusarium, Talaromyces, Clonostachys, etc. The fungal molecular ecological network exhibited a clear modular structure with a high ratio of positive correlation edges (99.33%), suggesting that positive cooperation was strengthen between different fungi. ( 2 ) FUNGuild analysis indicated that 10 guilds were highly correlated with yam tuber rot. Among these guilds, the relative abundances of dung saprotroph-undefined saprotroph-wood saprotroph and endophyte-plant pathogen were 33.74% and 23.64%, respectively, and the representative genera were Penicillium and Colletotrichum, respectively. Additionally, three guilds were related to both plant pathogen and wood saprotroph, and occupied 13.67% of the ecological functional groups. Moreover, Fusarium was representative genus of all three guilds. Traits analysis showed that some fungi (Penicillium, Fusarium, etc.) in the fungal community were probably invovled in yam tuber rot. ( 3 ) A total of 22 strains were isolated and belonged to 6 genera, including Fusarium ( 9 strains ), Penicillium ( 5 strains ) and Aspergillus ( 4 strains ), etc. This study provides an important reference for understanding the pathogenesis of tuber rot in Dioscorea sp., and for the selection of appropriate pesticides and biocontrol microbes.
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