Subjects: Biology >> Botany >> Applied botany submitted time 2023-07-26 Cooperative journals: 《广西植物》
Abstract： In order to select superior clones with strong resin-producing capacity and high resin-quality from the existing fast-growing timber clones in seed orchard of Pinus elliottii, and to make high use of crop germplasm resource,36 clones in the first generation of slash pine seed orchard were used as materials to determine their resin yield, resin mass flow rate and DBH growth, and to analyze their resin composition by GC-MS. Based on the above indicators, correlation analysis and cluster analysis was used to comprehensively evaluate the production and quality of resin among 36 clones. The results were as follows: (1) There were a total of 21 pine resin components, including 8 monoterpenes and 13 diterpenes. (2) Correlation analysis showed that the resin mass flow rate (RMR) had significant and positive correlation with the total content of monoterpene, weakly negatively correlates to abietic-type resin acid, and not significantly correlated to pimaric-type resin acid. (3) Based on the cluster analysis results integrating four types of indicators as the total monoterpene content, resin mass flow rate, abietic-type resin acid and pimaric-type resin acid, 36 clones could be divided into three categories and the difference between each type is significant. The performance of Class 1 was much better than that of the other two categories. (4) There were 17 high-resin yield pine clones (ERM≥15.15) among 36 clones, and on the basis of this, four clones (6-44, 4-11-1, 1-38 and 3-64) display higher monoterpenes content, while four clones (4-11-1, 3-64, 2-0420, 3-468) showed higher contents of pimaric-type resin acid. And the content of abietic-type resin acid of clone 2-173 was the highest. In summary, a total of 21 pine resin components of P. elliottii were identified, and 36 clones were evaluated based on four indicators: the total monoterpene content, resin mass flow rate, abietic-type resin acid and pimaric-type resin acid. We not only analyzed qualitatively the resin composition but also evaluated quantitatively the resin-producing capacity of 36 clones in slash pine seed orchard. Our findings provide the scientific references for the targeted breeding of pine resin components and lay a foundation for subsequent heredity breeding and gene improvement of P. elliottii.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-01-12 Cooperative journals: 《广西植物》
Abstract：为使速生湿地松良种快速大规模繁殖，对其胚性愈伤组织进行诱导和增殖优化研究。该研究以1代湿地松种子园中10个速生湿地松优良无性系（基因型）的未成熟合子胚为外植体，系统研究了基因型、合子胚发育阶段、基本培养基、植物生长调节剂种类和浓度等不同因子对胚性愈伤组织诱导效率的影响，并探讨了胚性愈伤组织的增殖条件。结果表明：基因型、合子胚发育阶段、基本培养基及植物生长调节剂种类和浓度等均对胚性愈伤组织诱导有不同程度的影响。10个基因型均诱导出了胚性愈伤组织，其中以2号基因型诱导率最高，达到25.37%；合子胚发育阶段中，以处于多胚阶段的球果诱导率最高；5种基本培养基中，以在DCR基本培养基上诱导效果最佳；植物生长调节剂浓度及组合中以2,4-D 2.0 mg·L-1 + KT 2.5 mg·L-1诱导率最高，达到27.78%。湿地松胚性愈伤组织增殖的最佳培养基为在DCR基本培养基上添加0.5 mg·L-1 2,4-D、1.0 mg·L-1 KT、500 mg·L-1 CH和300 mg·L-1 L-谷氨酰胺。该研究为湿地松良种进一步开展成熟胚诱导及植株再生奠定了基础。
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