Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-29 Cooperative journals: 《广西植物》
Abstract： Flavonoid-C- glycosides, as one of the effective components in Premna fulva, have the effect of antigen-induced arthritis. The enrichment and purification process of five flavonoid-C-glycosides as in P. fulva stems is preferred. Through static adsorption and desorption experiments on 13 kinds of macroporous resins with five flavonoid-C- glycosides in P. fulva stems as the index,a suitable macroporous resin was selected, and the results were tested by the HPLC, and then the orthogonal process was used to optimize the enrichment and purification conditions. The results showed that XAD-16N macroporous resin has a good adsorption and desorption effect on the five flavonoid -C-glycosides in P. fulva stems. Through single factor screening and orthogonal process optimization, the best enrichment and purification process is finally determined as follows: the loading concentration is 0.5 times the original extract diluted, the loading flow rate is 2 BV穐-1, and the eluent concentration is 60 % ethanol. The content of five flavonoid-C-glycosides increased from 1.73 % (in the original plant) to 14.5 %, an increase of 7.38 times. The results showed that the selected extraction process is stable and feasible, and the selected macroporous resin has a good separation and purification effect, which provides a reference for the drug development of P. fulva stems.
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