Subjects: Biology >> Botany >> Applied botany submitted time 2023-07-13 Cooperative journals: 《广西植物》
Abstract： Ardisia crenata var. bicolor is a common medicine used by Miao minority in Guizhou, which has the effects of clearing throat and benefiting pharynx, reducing swelling and relieving pain, dispelling wind and dehumidifying. In order to study the chemical constituents and anti-inflammatory activities of A. crenata var. bicolor roots, the 70% ethanol extact were separated and purified by silica gel, Sephadex LH-20 gel column chromatography, ODS reverse column chromatography and semi-preparative HPLC. The structures of the compounds were identified by spectral data of NMR, MS and published literatures. Using lipopolysaccharide(LPS)-activated RAW 264.7 cell line models in vitro, compounds 1-4 were evaluated for the inhibition against nitric oxide(NO) production. The results were as follows: Ten compounds were isolated from the ethanol extract and identified as 11-O-galloyl bergenin (1), 11-O-(4-O-methylgalloyl) bergenin (2), 11-O-vanilloylbergenin (3), 6-O-(4-hydroxy benzoyl) bergenin (4), 11-syringyl bergenin (5), 11-O-(3',4'-dimethylgalloyl)-bergenin (6), demethoxybergenin (7), micractinin A (8), monomethyl olivetol (9), and dibutyl phthalate (10). Among them, compounds 4, 8 and 9 were obtained from this genus for the first time. The results of anti-inflammatory activities in vitro were as follows: Compounds 1-4 could significantly inhibit NO release in RAW 264.7 cells (P < 0.01), and the inhibition rates of compounds at a concentration of 20 μmol·mL-1 were 67.09%, 66.50%, 59.83%, 36.47%, respectively. This study enriches the chemical constituents of this plant, clarify the material basis of its anti-inflammatory activities, verify the scientificity of its traditional usage, and provide new insight and scientific evidence for its rational development and utilization of the medicinal resources.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-07-20 Cooperative journals: 《广西植物》
Abstract： To study the salt tolerance mechanism of Hemerocallis minor and select excellent salt-tolerant plants, sand culture in different concentrations of NaCl (50, 100, 150, 200, 250 mmol·L-1) to explore the growth characteristics and related physiological indicators. The results were as follows: (1) 100-150 mmol·L-1 NaCl stress caused initial damage. Growth was significantly inhibited in a salty environment above 200 mmol·L-1. (2) NaCl stress above 200 mmol·L-1 caused the cell membrane permeability and MDA content increased greatly. (3) The content of proline in the leaves increased significantly with the NaCl concentration increase; Under 50-100 mmol·L-1 stress, the soluble sugar content increased at the initial stage then became decreased. The maximum appeared at 15th day; The slow change of soluble protein content in the leaves suggested it was not the main adjustment substance. H. minor could alleviate the salinity damage by improving cell membrane permeability and promoting the organic osmotic adjustment substances’ synthesis. For these reasons, H. minor could grow normally in saline-alkali soil below 50-150 mmol·L-1.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-05-11 Cooperative journals: 《广西植物》
Abstract： The dried roots of Sophora tonkinensis were commonly used as the treatment of the throat and other infectious diseases. In order to identify its large polar antibacterial active components, silica gel column chromatography (CC), ODS CC and Sephadex LH-20 CC and semi preparative HPLC were used to isolate the chemical constituents from n-butanol fraction of S. tonkinensis. The antibacterial activities of the monomers against bacteria (Enterobacter faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli) and fungi (Candida albicans) were evaluated. The results showed ten compounds were isolated and identified as 2',4',7-trihydroxy-6,8-bis(3-methyl-2-butenyl)flavanone(1),genistin(2), 2-2',4'-dihydroxy-5,6-dioxomethylbenzofuran(3), maltol-β-D-glucopyranoside(4), leonuriside A(5),coniferylalcohol-9-O-β-D-glucopyranoside(6),glabrol(7),2-(2',4'-dihydroxy-phenyl)-8,8-dimethyl-10-(3-methyl-2-butenyl)-8H-pyrano[2,3-d]chroman-4-one (8), oxymatrine (9), piscidic acid (10). Among of them, compound 4-6 was isolated from the title plant for the first time, for our knowledge. Besides, biological activity test showed the potential antibacterial activities of compounds 1-3. It is worth mentioning that the minimum inhibitory concentration (MIC) value of compound 2 against Pseudomonas aeruginosa was 15.6 μg·mL-1, while the MIC of compound 3 against Candida albicans and Escherichia coli were 31.3 μg·mL-1.
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