香合欢EST-SSR 标记开发及种间通用性研究

Subjects: Biology >> Botany >> Applied botany submitted time 2021-07-20 Cooperative journals: 《广西植物》

安琪 冯源恒 杨章旗 胡拉

Abstract： Albizia odoratissima is a unique rare timber tree specie in the south of China, and it is of great significance to carry out group genetics research on its germplasm resources, which is of great significance to the protection and utilization of all kinds of material resources of the tree. This study designs and develops EST-SSR markers of Albizia odoratissima based on the transcriptome sequencing results. In addition, Albizia procera, Albizzia falcata, Acacia melanoxylon, Erythrophloeum fordii and other related species were selected for analysis of interspecific generality. The results showed that among the 243 pairs of developed primers, 171 pairs could successfully amplify the target bands, and the effective amplification rate in Albizia odoratissima Albizia procera, Albizzia falcataria, Acacia melanoxylon and Erythrophloeum fordii was 63.79%, 33.75%, 45.68%, 41.56% and 14.81% respectively, and the polymorphism ratio in them was 23.87%, 12.20%, 9.01%, 3.96% and 2.78% respectively. There were 18 pairs of primers that could be commonly used among Albizia odoratissima, A. procera, A. falcataria, Acacia melanoxylon and Erythrophloeum fordii. There were 37 SSR polymorphism markers for Albizia odoratissima were obtained, 10 polymorphism markers for Albizia procera and A. falcataria, 4 polymorphism markers for Acacia melanoxylon, and there was 1 polymorphism mark of Erythrophloeum fordii. The developed EST-SSR markers can meet the needs of population genetic studies of Albizia odoratissima, and have good transferability and practicability in Albizia procera and A. falcataria and other related tree species. In conclusion, EST-SSR markers will provide a reliable research tool for genetic diversity evaluation of germplasm resources, fingerprint construction of breeding materials, and population mating system analysis of Albizia odoratissima, A. procera, A. falcataria, Acacia melanoxylon and Erythrophloeum fordii.

 Hits 838  Downloads 367  Comment 0