Subjects: Biology >> Botany >> Applied botany submitted time 2022-07-05 Cooperative journals: 《广西植物》
Abstract： The purpose of this paper was to study the material basis and the bioactive chemical components of Hypericum lagarocladum. The aerial part of this species was extracted with 80% ethanol, and then the crude extract was isolated and purified by HP-20 macroporous adsorption resin column chromatography (CC), silica gel CC, Sephadex LH-20 CC and semi-preparative HPLC. The structures of isolated compounds were deduced by the spectroscopic data, as well as comparison with the previous literature data. Fifteen compounds were purified from H. lagarocladum. They were identified as attenuatumione G (1), uralodin B (2), chipericumin C (3), 2,5-dihydroxy-1-methoxyxanthone(4),1,7-dihydroxyxanthone(5),1,7-dihydroxy-4-methoxyxanthone (6), quercitrin (7), apigenin-7-O-β-D-glucopyranoside (8), apigenin-7-O-β-D- (6''-O-acetyl)-glucopyranoside (9), luteolin (10), quercetin (11), betulinic acid (12), betulinic acid methyl ester (13), betulonic acid (14), and β-sitosterol (15). Compounds 1-14 are isolated from H. lagarocladum for the first time. In vitro cytotoxic activity of compounds 1-14 were evaluated using MTT method against MCF-7 and MDA-MB-231 cell lines. And only compounds 3, 6 and 13 showed weak cytotoxic activity with IC50 values ranging from 48.6 to 123.5 μg·mL-1. These research results provide a scientific theoretical basis of H. lagarocladum for its comprehensive development and utilization.
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