Subjects: Biology >> Botany >> Applied botany submitted time 2024-03-26 Cooperative journals: 《广西植物》
Abstract： Andrographis paniculata is one of the most important “Southern Medicines” in China. It is used for clearing heat and detoxifying, cooling blood and reducing swelling. Its main active ingredient, andrographolide, functions in anti-cancer, anti-HIV, anti-inflammation and liver protection. It is difficult to be synthesized artificially, mainly relying on extraction from cultivated plant materials. However, the quality of cultivated medicinal materials is affected by various factors such as soil, climate, water and fertilizer management. On the other hand, A. paniculata has a long growth cycle, occupying land resources. The technology of plant in vitro culture has significant advantages in rapid propagation of seedling and accumulation of active ingredients, which is one of the important ways to achieve production of active ingredients rapidly and efficiently in A. paniculata. The in vitro regeneration technology system of A. paniculata is becoming more and more perfect, and the in vitro regeneration technology from explants to complete plants is becoming more and more mature, it has been applied in seedling propagation and ploidy breeding. At the same time, during callus culture, cell suspension culture, adventitic root culture and hairy root culture of A. paniculata, the accumulation of andrographolide and other active ingredients in the culture things could be greatly increased by optimizing the culture conditions and using appropriate inducers. This paper comprehensively and systematically reviewed the research advances in the technology of in vitro culture of A. paniculata and production of andrographolide from the aspects of tissue culture, cell culture, adventitious root culture, and hairy root culture. This paper aimed to provide reference for promoting the development and application of in vitro culture technology of A. paniculata, as well as for the study of in vitro production of andrographolide. It also put forward three aspects that need be focused on in future research on in vitro culture technology of A. paniculata and the production of andrographolides by this technology: (1) to mature and improve the tissues in vitro regeneration technology system of A. paniculata, and establish a comprehensive and systematic evaluation system; (2) to further increase the yield of andrographolide and other important active ingredients by optimizing the culture conditions and its combination with efficient inducers; and (3) to carry out researches in bioreactor culture of the production of andrographolide by cell suspension culture technology.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-02-07 Cooperative journals: 《广西植物》
Abstract： Andrographis paniculata is one of the most important “Southern Medicines” in China. It is used for clearing heat and detoxifying, cooling blood and reducing swelling. Its main active ingredient, andrographolide, functions in anti-cancer, anti-HIV, anti-inflammation and liver protection. It is difficult to be synthesized artificially, mainly relying on extraction from cultivated plant materials. However, the quality of cultivated medicinal materials is affected by various factors such as soil, climate, water and fertilizer management. On the other hand, A. paniculata has a long growth cycle, occupying land resources. The technology of plant in vitro culture has significant advantages in rapid propagation of seedling and accumulation of active ingredients, which is one of the important ways to achieve production of active ingredients rapidly and efficiently in A. paniculata. The in vitro regeneration technology system of A. paniculata is becoming more and more perfect, and the in vitro regeneration technology from explants to complete plants is becoming more and more mature, it has been applied in seedling propagation and ploidy breeding. At the same time, during callus culture, cell suspension culture, adventitic root culture and hairy root culture of A. paniculata, the accumulation of andrographolide and other active ingredients in the culture things could be greatly increased by optimizing the culture conditions and using appropriate inducers. This paper comprehensively and systematically reviewed the research advances in the technology of in vitro culture of A. paniculata and production of andrographolide from the aspects of tissue culture, cell culture, adventitious root culture, and hairy root culture. This paper aimed to provide reference for promoting the development and application of in vitro culture technology of A. paniculata, as well as for the study of in vitro production of andrographolide. It also put forward three aspects that need be focused on in future research on in vitro culture technology of A. paniculata and the production of andrographolides by this technology: (1) to mature and improve the tissues in vitro regeneration technology system of A. paniculata, and establish a comprehensive and systematic evaluation system; (2) to further increase the yield of andrographolide and other important active ingredients by optimizing the culture conditions and its combination with efficient inducers; and (3) to carry out researches in bioreactor culture of the production of andrographolide by cell suspension culture technology.
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