Subjects: Biology >> Bioengineering submitted time 2017-11-21 Cooperative journals: 《中国生物工程杂志》
Abstract:为了解除微生物发酵生产丙酸过程中代谢产物(丙酸)对菌体生长的抑制作用,以实验室保藏的产酸丙酸杆菌(耐30g/L丙酸)为出发菌株P-0,通过丙酸压力筛选获得了一株耐10g/L丙酸的产酸性能良好的菌株P-10,降低了发酵过程中丙酸对菌体生长的抑制作用。菌株P-10做摇瓶发酵,发酵周期168h,丙酸浓度为49.66 g/L,产酸速率为0.30g/L·h,较出发菌株P-0提高了53.04%;7L发酵罐实验表明,菌株P-10发酵周期168h,丙酸浓度为55.63g/L,产酸速率0.33g/L·h。同时对菌株P-10做二次接种实验,结果表明,84h为二次接种最适时间段,且84h进行二次接种时,丙酸浓度提高了17.77%,二次接种实验不但有利于有机酸的积累,而且可以提高菌株的产酸能力和耐酸能力;经过选育的菌株P-10具有优良的产酸稳定性,有利于菌种的工业化生产和应用,同时对后续的发酵分离耦合具有重要意义。
Subjects: Biology >> Bioengineering submitted time 2017-11-21 Cooperative journals: 《中国生物工程杂志》
Abstract:为了解除微生物发酵生产丙酸过程中代谢产物(丙酸)对菌体生长的抑制作用,以实验室保藏的产酸丙酸杆菌(耐30g/L丙酸)为出发菌株P-0,通过丙酸压力筛选获得了一株耐10g/L丙酸的产酸性能良好的菌株P-10,降低了发酵过程中丙酸对菌体生长的抑制作用。菌株P-10做摇瓶发酵,发酵周期168h,丙酸浓度为49.66 g/L,产酸速率为0.30g/L·h,较出发菌株P-0提高了53.04%;7L发酵罐实验表明,菌株P-10发酵周期168h,丙酸浓度为55.63g/L,产酸速率0.33g/L·h。同时对菌株P-10做二次接种实验,结果表明,84h为二次接种最适时间段,且84h进行二次接种时,丙酸浓度提高了17.77%,二次接种实验不但有利于有机酸的积累,而且可以提高菌株的产酸能力和耐酸能力;经过选育的菌株P-10具有优良的产酸稳定性,有利于菌种的工业化生产和应用,同时对后续的发酵分离耦合具有重要意义。
Subjects: Biology >> Bioengineering submitted time 2017-09-20
Abstract: Chromatography has been playing an important role in bioseparation. As one of the main components of chromatography, the structure and properties of chromatographic media have great effects on purification results. Among them, capacity is one of the most important properties to determine chromatographic media’s purification effects, and how to improve the capacity is one of the key points having been paid close attention to in bioseparation. This paper is based on high-capacity agarose-based chromatographic media, with hydrophobic and immobilized metal ion affinity chromatographic media being tailor-made, respectively. For hydrophobic chromatographic media, various lengths of spacer arm were introduced with a controllable hydrophobic property, and the media was used in CHO-rHBsAg purification. rHBsAg binding capacity was greatly improved by this media containing spacer-arms, and rHBsAg recovery was more than 90% and the purification factor was 65.8, both higher than the results from traditional chromatographic media. For immobilized metal ion affinity chromatographic media, dextran molecules were grafted with manipulation and its capacity of His-tagged recombinant proteins was enhanced by 1.5 times than that of traditional media. This novel tailor-made high-capacity chromatographic media is promising for large scale purification of recombinant proteins.
Subjects: Biology >> Bioengineering submitted time 2017-07-24 Cooperative journals: 《中国生物工程杂志》
Abstract: Screening and Application of Acid-resistant Strains of Propionibacterium acidipropionici