同源过表达BglR对嗜热毁丝霉β-葡萄糖苷酶活性的影响

Subjects: Biology >> Bioengineering submitted time 2017-07-24 Cooperative journals: 《中国生物工程杂志》

来亚鹏 邓婷婷 刘刚 王娟

Abstract： Objective: This study focuses on the influence of overexpression of β-glucosidase regulator BglR on beta-glucosidase activities in Myceliophthora thermophila ATCC42464 by cloning bglr gene and constructing Mtbglr overexpression vector. Methods: The SLIC was adopted to construct Mtbglr overexpression vector and the promoter of MtPpdc and terminator of MtTpdc was used for overexpress bglr gene. The gene expression and β-glucosidase activities were observed by protoplast transformation, real-time quantitative PCR and enzymatic determination. Results: The bglr gene was overexpressed in M. thermophila successfully. The result showed that the β-glucosidase activity and secreted protein concentration of transformant strain Mt8 were 1.7 and 1.9 fold higher, respectively, than that of wild type WT. Conclusion: The expression of bglr increased the β-glucosidase activity in Myceliophthora thermophila under the inducing condition, which laid the theoretical foundation for the regulation of β-glucosidase gene of thermophilic fungi.

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