Subjects: Biology >> Botany >> Applied botany submitted time 2019-06-17 Cooperative journals: 《广西植物》
Abstract：非结构性碳水化合物（NSC）作为光合作用的主要产物，其含量多少可反映植物整体的碳收支平衡关系及对外界环境胁迫的适应策略。该文采用改进的苯酚-浓硫酸法对福建省平潭岛海岸处于生长旺盛期的3种典型沙生植物木麻黄、单叶蔓荆和老鼠艻茎叶的NSC及其组分含量进行测定，结果表明：（1）单叶蔓荆叶片、茎干可溶性糖含量均最大，分别为137.83±3.75 mg•g-1 和90.74±2.65 mg•g-1，老鼠艻叶片、茎干淀粉含量均最大，分别为105.41±2.49 mg•g-1和120.48±7.67 mg•g-1，二者茎叶NSC含量均大于木麻黄，且全部达到显著水平（P＜0.05）。（2）沙堆表面老鼠艻叶片NSC及组分含量从雏形、发育到稳定阶段变化不明显，但茎干NSC及组分含量在雏形阶段沙堆表面显著大于其它两个演替阶段（P＜0.05），且依次呈下降趋势。（3）从幼龄林、中龄林到成龄林阶段，木麻黄NSC含量呈现为先上升后下降趋势，其中成龄林叶片可溶性糖、淀粉和NSC含量、茎干可溶性糖和NSC含量均显著低于其它两个生长阶段（P＜0.05）。（4）不同沙生植物对环境胁迫的适应策略各异，海岸沙丘表面植被恢复应充分考虑物种特性及其更新。
Subjects: Biology >> Bioengineering submitted time 2018-12-16 Cooperative journals: 《中国生物工程杂志》
Abstract：目的 本研究主要是mir-219通过对TGFBR2调控机制，介导ENDMT途径缓解急性心肌梗死的发展。方法 qRT-PCR检测AMI患者及AMI小鼠血清中miR-219的表达量；过microRNA靶基因数据库TargetScantargetscan进行筛选预测；荧光素酶报告基因法及qRT-PCR分析TGFBR2与miR-219的调控机制；我们通过心脏超声心动图检测AMI小鼠心肌注射miR-219慢病毒4周后的血分数（LVEF）；采用qRT-PCR检测心肌注射miR-219慢病毒的AMI小鼠中Nppa 的mRNA的表达量；通过Masson’s trichrome染色法检测小鼠四周后左心室纤维化变化；使用α平滑肌肌动蛋白（α-SMA）对小鼠左室切片进行免疫组化分析；通过Western blot检测P-smad2、P-smad3及TGFBR2缺氧诱导蛋白磷酸化的表达水平。结果 miR-219对AMI有调控作用；miR-219可抑制TGFBR2的mRNA表达，而miR-219 inhibitor可以抑制这种下调效果，miR-219对TGFBR2具有抑制调控性；miR-219可抑制急性心肌梗死的进程，促进梗死心肌功能的恢复；miR-219能促进AMI小鼠心肌组织血管的新生和成熟，最终心脏收缩能力上升，心功能恢复；miR-219能抑制TGFBR2抑制EndMT途径，导致缓解AMI的病理进程。结论 miR-219能通过抑制TGFBR2影响EndMT途径，心肌纤维化减少，促进血管的新生和成熟，心功能恢复，抑制AMI的病理发展。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-09-07 Cooperative journals: 《广西植物》
Abstract： Quantification of non-structural carbohydrates (NSC) contents of plants is of great significance to the exploration of its growth and adaptive strategies. However, various pretreating methods often lead to the poor comparability among different results. In this paper, four typical psammophytes on the coast of South China including Casuarina equisetifolia, Spinifex littoreus, Ipomoea pes-caprae and Sesuvium portulacastrum were studied, and the effects of pretreating methods on the determination of non-structural carbohydrates contents of plants by phenol-concentrated sulfuric acid method were analyzed, including the existence of the peel and mesh size. The results showed that: (1) When peeled, NSC, soluble sugar and starch contents of the Casuarina equisetifolia samples all had a decreasing trend compared with the control group, especially significant in the soluble sugar contents of the young-aged forest and the middle-aged forest(P<0.05),butmade nodifference in both theirstarch contentsand all componentcontentsin adult ones. The smaller the age of samples, the greater the effect of peeling pretreatment on NSC andits componentcontentsin trees,especially affectedonsoluble sugar,so we suggestto preserve tree bark when samples processed. (2) The contents of NSC and its components of different psammophytes’s branches (stem) all raised with the increase of mesh size, and there were significant differences between the Casuarina equisetifolia and Spinifex littoreus’branches (stem) samples which passed through the 100 and 18 mesh sieve (P<0.05). Except the Spinifex littoreus, all psammophytes’ leaves (assimilating branches) samples hadn’t changed much under different mush sizes and had no significant difference. The higher the content of mechanical tissue in the samples, the more significant difference in the content of NSC and its components extracted by different mesh size, considering the difficulty in obtaining samples and the accuracy of the determination of NSC content, thus we suggest that all branches and leaves of psammophytes should be processed by 100 mesh sieve. The conclusion of this study can guide the sample processing methods of related research in South China Coast, standardize experimental methods andimprove thecomparabilitybetweendifferentstudies.
Subjects: Biology >> Zoology submitted time 2017-10-11 Cooperative journals: 《动物营养学报》
Abstract：本研究探讨了饲粮中添加鹅脱氧胆汁酸（CDCA）对种鸡脂代谢、生产性能及子代肌肉发育的影响。试验选用14周龄矮脚黄种鸡80只，随机分为5个组，每组16个重复，每个重复1只鸡。对照组饲喂基础饲粮，试验组在基础饲粮中分别添加10、50、100和500 mg/kg的CDCA，试验期4周。在试验最后1周收集种蛋进行孵化，出雏后的子代根据种鸡分组情况分为5组，饲养期12周。结果表明：1）饲粮中添加CDCA对种鸡的体重、产蛋率和蛋品质均无显著影响（P>0.05）；与对照组相比，饲粮中添加100 mg/kg的CDCA能够显著增加种鸡血清中甘油三酯（TG）含量（P<0.05），显著降低血清中极低密度脂蛋白（VLDL）含量（P<0.05）；与对照组相比，饲粮中添加100 mg/kg的CDCA能够显著降低试验第14天和第28天种鸡蛋内总胆汁酸（TBA）含量（P<0.05），显著增加蛋内胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）含量（P<0.05）。2）与对照组相比，饲粮中添加50 mg/kg的CDCA能够显著增加子代腓肠肌肌纤维密度和子代公鸡肝脏率（P<0.05）；与对照组相比，饲粮中添加100 mg/kg的CDCA能够显著降低子代母鸡的平均日增重、子代公鸡腓肠肌总蛋白沉积量和子代母鸡腓肠肌中乳酸脱氢酶（LDH）活性（P<0.05）；与对照组相比，饲粮中添加500 mg/kg的CDCA能够显著降低子代母鸡腓肠肌琥珀酸脱氢酶（SDH）活性和子代公鸡腓肠肌LDH活性（P<0.05）。由此可见，饲粮中添加CDCA对种鸡的生产性能无显著影响，但会影响种鸡的脂代谢以及蛋内沉积特别是TBA的沉积，进而对子代肌肉发育产生抑制作用，因此CDCA在种鸡饲粮中的应用应注意添加剂量以及使用时间。
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