Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： The classification of Mussaenda L. has been controversial due to the complex interspecific variation and hybridization within the genus, by exploring the variation patterns and taxonomical significance of the leaf venation characteristics between Mussaenda L. and its relative genera (Schizomussaenda Li and Psudomussaenda Wernham.), it could be provide data for the identification and utilization when they were regarded as the medical and garden plant resources. The leaf venation characteristics of 22 species of this group were observed by using the cleaning method. The cluster analysis was carried out based on the characteristics of leaf venation, and a classification retrieval table of the species was compiled. The results were as follows: (1) The characteristics of the leaf venation of Mussaenda L. and its relative genera had consistency, which were mainly reflected on that the primary veins were all pinnate, the frequency of intersecondary veins was less than one per areolation, the angle between the major secondary veins and the midvein was acute, the tertiary veins were mostly penetrating, and the arrangement of the areolation were all irregular. (2) The characteristics of the leaf venation with taxonomic value among genera or species within genera included the type of major secondary vein venation, the angle between the major secondary vein and the midvein, the spacing between the major secondary vein and the midvein, the connection between the major secondary vein and the midvein, the existence of inter-secondary veins and intramarginal secondary veins, the penetration of intercostal tertiary veins, the type of quaternary veins, the branching of freely ending veinlets, and the development of areolation. (3) According to the cluster analysis, the 22 species were clustered into 12 branches, and Schizomussaenda and Psudomussaenda were not clustered into one branch respectively, showing a relatively close genetic relationship. In conclusion, the leaf venation characteristics of Mussaenda and its related taxa can provide new information for the classification of the plants.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-05-21 Cooperative journals: 《广西植物》
Abstract： Legumes are abundant in the nitrogen deficient desert ecosystems, which is the center of supplying available nitrogen and the important pioneer species in this region. However, the studies on nitrogen uptake and utilization by leguminous in desert ecosystems have rarely been reported so far. In this study Astragalus flexus and Astragalus arpilobus, which are widely distributed in the Gurbantunggut desert, were selected as the research object. Three different nitrogen forms (15N-NH4 +、15N-NO3 -、15N-Glycine) were added to 0 ~ 5 and 5 ~ 15 cm soil layers, respectively, to study the absorption and distribution strategies of different forms of nitrogen in two plants and their organs. The results showed that: (1) In different soil layers, both plants preferred to absorb nitrate nitrogen, and the maximum absorption rates of Astragalus flexus and Astragalus arpilobus were 3.26、2.59 μg · g–1 · h–1, respectively. (2) In different soil layers, there were significant differences in the uptake and allocation of different nitrogen sources among plant organs (P < 0.05). The uptake of 15N in root of Astragalus flexus was higher than that of Astragalus arpilobus, and the three different nitrogen forms were mainly allocated to leaves. (3) In different soil layers, the contribution rates of different nitrogen sources to both plants were was 15N-NO3 - > 15N-Glycine > 15N-NH4 +, and the contribution rates of nitrate nitrogen to nitrogen absorption of Astragalus flexus were ranged from 37% to 41%, while the maximum contribution rate to the nitrogen absorption of Astragalus arpilobus was 45%. (4) There were significant differences in recovery rates of different nitrogen forms between different organs of plants (P < 0.05). In the 0 ~ 5 cm soil layer, the recovery rate of nitrate nitrogen was leaf > stem > root, while in the 5 ~ 15 cm soil layer, the rate was leaf > root > stem. In general, in the Gurbantunggut desert ecosystem, different life forms legumes had both consistency and difference in nitrogen uptake and allocation capacity, and were affected by different soil depths and nitrogen forms. The results provided theoretical basis for nitrogen uptake, utilization and allocation of legumes in arid and semi-arid regions of Xinjiang.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-08-09 Cooperative journals: 《广西植物》
Abstract： Under drought and NaCl stresses, the plant growth and development are affected significantly. In this study, to reveal the adaptability of Lycium ruthenicum to salt-drought stresses, the paper used pot culture test method to study the effects of salt and simulated drought interaction experiments on seedling growth, and observe the physiological responses by spraying different concentrations of salicylic acid that improve the survival rate of L. ruthenicum seedlings under salt-drought stress. The results showed that were significantly increased from control in the contents of soluble sugar, soluble protein and proline(Pro) of L. ruthenicum treated with exogenous SA under intercross salt-drought stress in the leaves, the malondialdehyede(MDA) content decreased(P＜0.05), whereas increased the activities of catalase(CAT), peroxidase(POD), and superoxide(SOD), among all concentrations, 0.5 mmol·L-1 SA treatment had better effect than 0.1 mmol·L-1 SA. To sum up, L. ruthenicum have a certain ability to adapt mild intercross of salt and drought, moderate concentrations of SA could improve the osmotic regulatory substances and antioxidant enzymes activities in the leaves under salt-drought environment. The study results can provide the theoretical bases for deeply understanding the growing of L. ruthenicum under different stresses．
Subjects: Biology >> Botany >> Applied botany submitted time 2020-03-06 Cooperative journals: 《广西植物》
Abstract：该研究从DNA 模板浓度、引物浓度、退火温度和循环次数等方面优化了叶子花ISSR-PCR 反应体系和反应程序，利用11 个ISSR 引物对131 个叶子花品种进行PCR 扩增，扩增产物经琼脂糖凝胶电泳检测，分析品种遗传多样性和遗传距离，并构建品种聚类图和指纹图谱。结果表明，优化的ISSR-PCR 反应体系中DNA 模板浓度为0.5 ng·μL-1，引物浓度为0.5 μM·L-1，引物UBC813、UBC814、UBC815、UBC823、UBC824、UBC835、UBC840、UBC841、UBC843、UBC844 和UBC876 的最佳退火温度分别为52.3、55.9、54.3、54.3、53.6、56.2、56.2、51.9、54.4、54、50 ℃，循环次数为32。用11 个ISSR 引物对131 个叶子花品种扩增出161 条带，其中多态性条带156 条，多态性比率为96.89%。单个引物的等位基因数、有效等位基因数、Nei’s 基因多样性指数和Shannon’s信息指数分别为1.86~2.00、1.33~1.68、0.21~0.39 和0.34~0.57，平均值分别为1.969、1.478、0.294 和0.447。引物UBC841 的鉴别率最高(80.92%)，可有效鉴别106 个品种，再与引物UBC876 结合可将131 个叶子花品种完全鉴别开，建立了各品种的指纹图谱。叶子花品种的遗传距离范围为0.00~0.60，平均值0.365，遗传多样性较低，在遗传距离0.58 处，131 个品种分为6 大类群，聚类分析表明同一个种的品种大多数聚在一类，但同一个种仍有品种未聚在一类或亚类、也有多个种的品种聚在一类。该研究较为准确的揭示了叶子花种质资源的遗传多样性，建立的指纹图谱为叶子花品种登记、知识产权保护以及品种鉴定提供了可靠技术和有效手段。
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