Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-13 Cooperative journals: 《广西植物》
Abstract： Bryophytes are important indicators of environmental pollution. However, bryophytes are mainly used to monitor atmospheric pollution in China. In fact, bryophytes have been widely applied to monitor water quality abroad. In the present paper, we firstly analyzed the literature of applying bryophytes to monitor water environmental quality abroad, then summarized relevant content of bryomonitoring, which included the types of water pollutants monitored by bryophytes, the species of bryophytes used for bryomonitoring, the influences of the survival status of bryophytes on monitoring effect, the environmental factors affecting the enrichment of heavy metal elements in water by bryophytes, the active and passive methods of applying bryophytes to monitor water pollutions, and the sample preparation, as well as some European cases of bryomonitoring for water environmental quality. Finally we also proposed some suggestions about bryomonitoring researches and application in the future.
Subjects: Biology >> Bioengineering submitted time 2018-03-22 Cooperative journals: 《中国生物工程杂志》
Abstract：研究人类线粒体肌酸激酶uMtCK的结合位点，将其与底物肌酸和ATP结合有关的关键氨基酸进行突变，并对突变体进行酶动力学和圆二色谱数据分析，探讨这些关键氨基酸在底物识别和催化过程中的作用。结果显示，与野生酶相比，突变体Q313A和R336A的 KmCr分别提高了2.6和2.9倍，kcat下降了19%和55%；同样地，与ATP结合相关的突变体R125A和R287A分别使得KmATP升高了3.2和4.2，kcat下降了72%和38%。以上结果表明突变体R125A、R287A、Q313A和R336A影响对底物的结合，同时也降低了酶促反应的速度。利用圆二色谱比较野生酶与不同突变体的二级结构并无明显变化，但进一步的结构模拟表明底物结合位点氨基酸在与底物之间的氢键对底物的识别和酶催化过程中发挥着重要作用。
Subjects: Biology >> Bioengineering submitted time 2017-07-24 Cooperative journals: 《中国生物工程杂志》
Abstract：骆驼体内存在天然缺失轻链的重链抗体（HcAb），其单域抗原结合片段也称为VHH或纳米抗体（nanobody，Nb），是目前已知的最小抗原结合片段。与传统抗体相比，纳米抗体具有分子量小（12-15KDa）、稳定性好和免疫原性低等特点，这些特点使得VHH在基础研究、诊断及治疗上具有极大的应用价值，目前已有多种纳米抗体进入了临床研究阶段。本文综述了近年来VHH在革兰氏阴性菌（大肠杆菌）、革兰氏阳性菌（芽孢杆菌和乳酸杆菌）、酵母、丝状真菌、昆虫细胞、哺乳动物细胞和植物细胞中异源表达的研究现状，包括表达系统、宿主、载体特点、载体构建方式及产量等；从分子水平、表达水平和理性设计三个层面探讨了纳米抗体产量提高的策略，希望为纳米抗体同行研究者提供一些借鉴和思路。
Hits
YES
Hits
Downloads
Comment