Subjects: Biology >> Botany >> Applied botany submitted time 2023-08-04 Cooperative journals: 《广西植物》
Abstract： Drought and land salinization are inhibiting factors for the sustainable development of forestry, and the plants will release volatile substances such as terpenoids during life or non-biological stress. In order to investigate whether HDR gene of Pinus massoniana is involved in stress response under drought and salt stress, the open reading frame of PmHDR gene was cloned, and bioinformation, tissue specific expression and preliminary function were analyzed. The results were as follows: (1) The coding region of PmHDR gene is 1 458 bp, encoding 485 amino acids, and its encoded protein contains the core sequence of LytB/IspH gene superfamily and PLN02821 multifunctional structural domain, which belongs to the 1-hydroxy-2-methyl-2-(E)-butenyl-4-pyrophosphate reductase family. (2) The PmHDR codon use preference was weak, with a preference for codons ending in A/U. Nicotiana tabacum, Arabidopsis thaliana and Saccharomyces cerevisiae were more suitable as its heterologous expression receptors. (3) qRT-PCR showed that the PmHDR gene was most highly expressed in old needles of Pinus massoniana, followed by young needles, young stems and old stems and least expressed in roots. (4) The gene expression vector pBI121-PmHDR was constructed and transformed into Arabidopsis thaliana. The transgenic A. thaliana showed greater resistance to drought and salt stress. These results indicate that PmHDR is involved in plant response and regulation to drought and salt stress, and these results can provide some theoretical support for Pinus massoniana stress-resistance breeding.
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