Extraction, purification and biological activity of total triterpenes from Sanghuangporus sanghuang

Subjects: Biology >> Botany >> Applied botany submitted time 2024-08-08 Cooperative journals: 《广西植物》

LIU Kun FU Xinyao GUO Tong ZHANG Wanyue ZHAO Qingsheng KONG Zejuan CHENG Hua SONG Heng

Abstract： Aim to optimize the extraction process of total triterpenes from Sanghuangporus sanghuang, purify the total triterpenes, analyze its chemical composition, as well as its antitumor and antioxidant activities. This study first used the response surface method to optimize the extraction process of total triterpenes from S. sanghuang. Secondly, D101 macroporous resin and Sephadex LH-20 chromatography were used to purify the total triterpenes. Meanwhile UHPLC-ESI-MS technology was used to analyze the composition of triterpenes, Finally, the anti-tumor and antioxidant activities of total triterpenes were studied by the methods of CCK8, DPPH and ABTS. The results were as follows: (1) The optimal conditions were extraction time 55, ultrasonic temperature 45 ℃, ethanol concentration 75% and a liquid-solid ratio of 35:1 (mL·g-1). Under these conditions, the total triterpene content was 10.34 mg·g-1. (2) The purity of Fraction a (Fa) was increased by 3.8 times to 47.98%. Eight triterpene components were identified from Fa: glycyrrhetinic acid, xibutanone, ursolic acid, euphorbiol, 24, 25-dihydrolanosterol, soya saponin B, lanosterol and cratonic acid. (3) The inhibition rate of Fa on PC3 was 68.65% at 200 μg·mL-1, which was higher than that of 5-fluorouracil (66.3%). Fa exhibited high DPPH and ABTS radical-scavenging activities with IC50 values of 42.76 and 66.24 μg·mL-1, respectively. This study provides a theoretical foundation for further research and utilization of total triterpenes from S. sanghuang.

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