Asymbiotic germination and rapid propagation of Paphiopedilum GXAAS Prince postprint

Abstract: Paphiopedilum GXAAS Prince is the first new variety of Paphiopedilum in China to obtain National Plant Variety Protection Right. Characterized by its elegant flower form, pure coloration, and extended blooming period, this cultivar serves as an exemplary representative of small to medium-sized solid-colored Paphiopedilum potted plants and enjoys exceptional market acceptance. To address the bottleneck in the industrial propagation of P. GXAAS Prince, this study employed asymbiotic germination and tissue culture techniques to identify the key factors influencing its seed asymbiotic germination and plant regeneration, and established an efficient and stable rapid propagation technology system. Using seeds at different developmental stages (150–330 days post-pollination) as explants, we systematically investigated the effects of seed maturity on germination rate and time. Through medium screening experiments, we optimized the medium formulations for protocorm induction, proliferation and differentiation, as well as rooted plantlet development. Results were as follows: (1) The optimal capsule harvest