Abstract:
Identification of chemical components in seed coat of rare tree species Ormosia henryi and quantitative analysis of endogenous abscisic acid XIN Tianze1,2, KUANG Yuanwen1,2, LE Xingui3, XU Bingqiang1, LI Hanxiang1* (1. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 516050, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. Yangjifeng National Nature Reserve Administration, Guixi 33500, Jiangxi, China) The seed coat of Ormosia henryi, a rare species in the genus Ormosia, possesses important values in timber, ornamental, and medicinal uses. However, its wild resources are declining due to low seed germination rate, over-exploitation, and difficulties in natural regeneration. To clarify the chemical composition of the seed coat and provide basic information for exploring potential chemical regulation factors during seed germination, metabolomic analysis was conducted on the seed coat of Ormosia henryi using UPLC-LTQ-Orbitrap MS. The structures of compounds were identified based on accurate molecular mass, characteristic fragment ions, MS/MS fragmentation patterns, and comparison with reference standards and literature data. Moreover, endogenous abscisic acid (ABA) content in the seed coat was quantitatively determined using multiple reaction monitoring (MRM) mass spectrometry. The results showed that: (1) 31 compounds were identified from the seed coat, including 19 flavonoids, 2 anthraquinones, 2 alkaloids, 3 terpenoids, 2 amino acids, and 3 organic acids. (2) Endogenous abscisic acid was detected in the seed coat with a content of (89.5 ± 0.3) μg·g⁻¹. The identification of chemical components and quantitative characterization of endogenous ABA in the seed coat of Ormosia henryi provide reference for further understanding the physiological characteristics related to seed germination and artificial propagation of rare tree species.
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