Bioinformatics analysis and expressionof PmWRKY2 and PmWRKY6 in Pinus massoniana postprint

Abstract: The WRKY family is one of the largest families of transcription factors in higher plants. Previous studies have found that WRKY family genes play an important role in the response of Pinus massoniana to insect pests, growth and development, and drought stress. In order to further study the biological function of WRKY family genes and the response to exogenous hormones, the bioinformatics characteristics of PmWRKY2 and PmWRKY6 genes were analyzed, and the expression patterns of PmWRKY2 and PmWRKY6 genes under exogenous hormones and calcium ion treatment such as ABA, SA, MeJA and GA were studied by qPCR. The results were as follows: (1) PmWRKY2 and PmWRKY6 proteins encoded 667 and 575 amino acids, respectively, and the subcells were located in the nucleus, and the N-terminus of the two genes contained a highly conserved WRKYGQK heptapeptide structure, and the C-terminus contained a zinc finger structure, which belonged to the WRKY transcription factor family. (2) In terms of evolutionary relationship, PmWRKY2 protein and PmWRKY6 protein were most closely related to PtXG20020.1 and Pt2G29990.1 proteins, which were also gymnosperm Pinus tabuliformis. (3) Compared with CK, the expression of PmWRKY2 and PmWRKY6 genes could be significantly induced by the four hormone treatments and the corresponding Ca2+ treatment, and their expression levels reached the peak in the late treatment period. The expression of PmWRKY2 gene was significantly induced in the early stage of Ca2+ treatment, and there was no significant difference between the expression of PmWRKY2 gene in the late treatment and the control, while the expression level of PmWRKY6 gene was significantly higher than that of the control during the whole treatment. These results show that both PmWRKY2 and PmWRKY6 genes can respond to different exogenous signaling substances, but the expression patterns are different.