Phenotypic traits and SSR molecular identification of hybrid progenies of Camellia chekiangoleosa × C. semiserrata 后印本

Abstract: Camellia chekiangoleosa has high oil content and oleic acid content, while Camellia semiserrata has strong growth vigor and resistance. In order to take advantage of the advantages of C. chekiangoleosa and C. semiserrata and cultivate excellent germplasm materials, In this paper, the phenotypic traits of 45 F1 hybrid progenies of C. chekiangoleosa and C. semiserrata were analyzed to grasp the phenotypic traits of the hybrids, in addition, SSR markers were used to identify hybrids, and SSR markers that could be used to identify the hybrids of oil tea were screened. The results were as follows：(1) The F1 hybrids of C. chekiangoleosa×C. semiserrata showed tall tree and rapid growth, and their leaf veins, sepals and stigmas were all tended to the traits of the male parent C. semiserrata, while flower and leaf morphology and other traits were similar to the female parent C. chekiangoleosa, and the characteristics of leaf color and size were between those of the parents. (2) From the 32 SSR markers, 8 fully complementary markers that can distinguish parents and determine the origin of offspring were screened out for identification of hybrids, among which the hybrid identification rate of 7 markers was as high as 100%, and the hybrid identification rate of 1 marker was 55.56%. 45 hybrids were all true hybrids identified by the complementarity of 8 markers. (3) The 8 SSR markers were used to verify the ability to identify the hybrids, indicating that it was feasible to use these SSR markers to identify the authenticity of the hybrids of oil tea. This study provide a reference for interspecies cross breeding of oil tea, and also provide a basis for the SSR marker identification of hybrids of oil tea.