Subjects: Biology >> Biochemistry submitted time 2024-04-24
Abstract： How to differentiate the diagnosis of depression and bipolar disorder has always been an important problem that needs to be solved urgently in clinical practice. In this study, from the perspective of urine proteomics, urine samples of similar age were collected from two hospitals to investigate the candidate biomarkers for differentiating the diagnosis of depression and bipolar disorder using both group analysis and one-to-many analysis. The experimental results of the paired group analysis showed that 108 differential proteins were identified in the depressed group compared to the bipolar group under strict screening conditions with screening criteria of FC ≥ 2 or ≤ 0.5 and a two-tailed unpaired t-test of P < 0.01, with an average of 3.7 randomly generated differential proteins, and a confidence level of 96.6 % for the correlation between these proteins and the disease difference. In the one-to-many analysis, 24 differential proteins were co-identified by the samples of 13 depressed patients, 16 of which showed a completely consistent trend of expression changes in all depressed patients studied, and 6 of which were associated with immunoglobulins; 41 differential proteins were co-identified by the samples of 12 depressed patients out of 13, and 19 of which showed a completely consistent trend of expression change in the These results reflect the strong consistency of differential proteins between the two groups of patients. 12 or more samples from depressed patients were enriched for differential proteins related to multiple biological processes and signaling pathways associated with the immune system, which is consistent with previous studies: immune mechanisms may be one of the pathogenetic mechanisms of major depression and that drugs with major immune targets can improve depressive symptoms. In the future, it may be possible to observe the immune status of patients with depression to provide direction and basis for the precise treatment of depression. The results of this paper show that urine proteomics can differentiate between depression and bipolar disorder, suggest possible mechanisms and potential targets for the treatment of depression and bipolar disorder, and provide a tool for future differential diagnosis and precision treatment of the diseases.
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Awaiting Review
Subjects: Biology >> Biochemistry Subjects: Biology >> Molecular Biology submitted time 2024-01-25
Abstract： Objective: To explore the possible effects of different sweet taste substances on the body by analyzing the changes of urinary proteome in mice after self-consumption of different sweet taste substances.
Methods: Urine samples of C57BL/6l mice were collected before and after self-consumption of sweet substances, including sucrose, stevia glycosides, acesulfame and sucralose, which are widely used in the world and can cause the preference reaction of mice. Among them, the concentration of non-nutritive sweeteners was selected as the concentration that has been shown to have the strongest preference reaction of mice. Label-free quantitative proteomics using high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for analysis. Differential proteins of urine proteome were screened in groups for analysis of protein functions and biological pathways. The urine proteome of single mice before and after self-consumption of sweet substances was compared, and the common differential proteins were counted; and the different sweet substances were compared horizontally.
Results and conclusions: Urine proteome can reflect the body changes of mice after self-consumption of sweet substances. And the effects of different sweet substances on urine proteome were different. Among the four sweet substances, sucralose caused the most similar changes in the body compared with sucrose. Compared with sucrose, stevia glycosides caused the most different changes in the body. The body changes caused by sucrose, acesulfame and sucralose are similar, but the body changes caused by stevia glycoside are different from other sweet substances. After self-consumption of the four sweet substances, the urine proteome differential proteins in mice all had proteins that had been reported to be related to brain reward circuitry, while only the urine proteome differential proteins after self-consumption of sucrose, acesulfame and sucralose were mainly related to metabolic processes. Urine proteomic differential proteins after acesulfame of stevia glycoside were mainly related to nucleosome assembly and gene expression.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2024-01-20
Abstract： Do rats have corresponding changes in their urinary proteome when smelling odors? Do sniffing different odors produce different changes? In this study, urine samples were collected from six rats after smelling sesame oil and essential balm for three days, and on the third and fourth days, and the samples were analyzed in groups and single before and after comparisons using LC-MS/MS technology. The identified differential proteins were also compared with those produced by the growth and development of rats of the same age group to exclude the influence of growth and development on the results of this experiment. The experimental results showed that comparing the urine protein groups of Day0 and Day4 of the sesame oil group, 143 differential proteins could be identified after screening, and the average number of randomly generated differential proteins was 7.32, at least 94.88% of the differential proteins were not randomly generated. Upon comparative analysis in groups and singly before and after, the same odor showed more consistent changes, and differential proteins such as low-density lipoprotein receptor-related protein 2 and fetuin B, a biomarker of COPD, which are associated with olfactory production, were identified in the sesame oil group, while uteroglobulin, trichothecene factor 3, and visfatin 2 were identified in the essential balm group, which had significant changes and were related to the production of olfactory sensation. It is noteworthy that we identified odor-binding protein 2A again in the essential balm group, simultaneously present in the differential proteins produced by a single before-and-after comparison in four rats, which is consistent with the results presented in the e-cigarette model. This study demonstrates that odor affects the urinary proteome of rats, with different odors affecting it differently. This provides a new approach to explore the biological process of olfaction.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2024-01-19
Abstract： Zinc is an essential element for maintaining normal physiological function in living organisms. In this study, 82 mg/kg·d zinc gluconate (equivalent to 11.7 mg/kg·d zinc) was intragastrically administered to rats for 4 days, and the urine proteome of rats before and after short-term intragastric administration of zinc gluconate was compared and analyzed. Many differential proteins have been reported to be zinc related, such as mucin-2 (MUC-2) (14 times before compared with after gavage, p = 0.005) and transthyretin (3.9 times after gavage compared with before gavage, p = 0.0004). Biological processes enriched in differential proteins (e.g., regulation of apoptosis process, immune system process, etc.), molecular functions (e.g., calcium binding, copper binding, signaling receptor activity, etc.), KEGG pathways (e.g., complement and coagulation cascades, PI3K-Akt signaling pathway, etc.) showed correlation with zinc. In this study, we explore the overall effect of zinc on the body from the perspective of urine proteomics, which is helpful to deeply understand the biological function of zinc and broaden the application potential of urine proteomics.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2024-01-18
Abstract： Calcium is an essential element for maintaining the normal physiological function of organisms. In this study, 3225 mg/kg · d calcium gluconate (equivalent to 300 mg/kg · d calcium) was intragastrically administered to rats for 4 days, and the urine proteome of rats was analyzed. Many differential proteins have been reported to be calcium related, such as Regucalcin (2.6 times higher after gavage than before gavage, p = 0.022), transmembrane protein 132A (8.2 times higher after gavage than before gavage, p = 0.009), creatine kinase (17.5 times higher before gavage than after gavage, p = 0.006), and claudin-3 (13.3 times higher before gavage than after gavage, p = 0.037). Differential protein enriched KEGG pathways included calcium signaling pathways, and biological processes and molecular functions also showed correlation with calcium. In this study, from the perspective of urine proteomics to explore the overall impact of calcium on the body, it is helpful to deeply understand the biological function of calcium and broaden the application potential of urine proteomics.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2024-01-04
Abstract： Iron is an essential trace element to maintain the normal physiological function of organisms. No studies have investigated the overall effect of iron on the body from the perspective of urine proteome. In this study, the urine proteome of rats before and after short-term intragastric administration of polysaccharide-iron complex (28mg/kg·d iron, which is equivalent to the dose of anemia prevention in adults) was compared and analyzed by using two analysis methods: individual comparison and group comparison. Many different proteins were reported to be related to iron, including 2', 3' -cyclic nucleotide 3' -phosphodiesterase (CNPase) (7.7 times higher than that after gavage, p=0.0039), p38 (14.5 times higher than that before gavage, p=0.003), etc. In the individual comparison, Hepcidin was up-regulated in 4 rats simultaneously. The biological processes of differential protein enrichment include carbohydrate metabolism, iron ion reaction, apoptosis regulation, hematopoietic progenitor cell differentiation, etc. Molecular functions (e.g., complement binding, hemoglobin binding, etc.), KEGG pathways (e.g., complement and coagulation cascade, cholesterol metabolism, malaria, etc.) have also been shown to be associated with iron. This study contributes to the in-depth understanding of the biological function of iron from the perspective of urine proteomics, and provides a new research perspective for the prevention, diagnosis, treatment and monitoring of iron-related disorders.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry Subjects: Biology >> Molecular Biology submitted time 2024-01-02
Abstract： Objective: Comparison of urine proteome between obese people and normal people.
Methods: Urine samples from obese and normal people were collected and identified by non-label quantitative proteomics using high performance liquid chromatography tandem mass spectrometry (LC-MS/MS). The difference proteins of urine proteome between obese and normal people were screened for protein function and biological pathway analysis. The urine proteome of obese individuals was compared with that of normal people, and the common differential proteins were counted to analyze the protein function and biological pathways. Reported biomarkers of obesity were searched in the urine proteome of obese individuals.
Results: 38 different proteins can be identified in the urine proteome of obese people compared with normal people, some of which have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. 8 common differential proteins in the urine proteome of obese individuals and normal people, among which some proteins have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. Among the differential proteins in the urine proteome of obese individuals compared with the normal people, the reported obesity biomarkers can be matched.
Conclusions: The urine proteome can distinguish the obese people, and the differential proteins in the urine proteome have key proteins that are known to be related to obesity and metabolism, and the biological processes of differential proteins also related biological processes such as nutrition and metabolism. Urine proteome has the potential to explore the pathogenesis of obesity and provide personalized treatment.
Peer Review Status:Awaiting Review
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