分类： 生物学 >> 生物医药 分类： 生物学 >> 病毒学 提交时间： 2017-07-11
摘要： Objective: Viral clearance of human HBV infection largely depends on the age of exposure, so a mouse model with age-dependent immune response and immune-tolerance for HBV infection is essential. Methods: HBVRag1 mice were generated by crossing Rag1-/- mice with HBV-Tg mice. The differences between adult and young HBVRag1 mice were detected after adoptive transfer of splenocytes. Immune tolerance was evaluated by quantitative hepatitis B core antibody (qAnti-HBc) assay, adoptive transfer, and modulation of gut microbiota with antibiotic. Results: After HBVRag1 mouse reconstitution, adult mice showed obvious HBV-dependent inflammation and hepatocytes damage, cleared HBsAg and generated HBsAb and HBcAb, but young mice never developed ALT elevation, and only generated HBcAb with persistence of HBsAg. In addition, for adult mice, more hepatic CD8+T and B cells promoted clearance of HBsAg 30 days after lymphocytes transfer, and for young mice, higher levels of cytokines link to the persistence of viral antigens during initiation of immune response towards HBV. The level of qAnti-HBc increased significantly with the time of adoptive transfer in young mice, but decreased significantly in adult within our model. This mimics kinetic changes of human HBV infection regarding qAnti-HBc level. Also, the age-related tolerance in this model was different from which was in HBV-Tg mice, and can be regulated through modulation of gut microbiota. Meanwhile, GS-9620 can achieve inhibition of HBsAg, but HBV vaccine just clears limited HBsAg within the model. Conclusions: Here, we described a mouse model with age-dependent immune response and immune tolerance of HBV infection which could mimic chronic HBV infection in human. It will open a door for evaluating new therapeutic approaches before clinical trials.
分类： 生物学 >> 生物工程 分类： 生物学 >> 生物医药 分类： 物理学 >> 电磁学、光学、声学、传热、经典力学和流体动力学 提交时间： 2017-03-17
摘要： [目的] 临床观察脑功能康复仪（脑康仪）对脑循环和脑功能的影响。[方法] 将脑血管病患者180例，分为脑梗塞组、脑动脉硬化症组、椎-基底动脉供血不足组，每组各60例，按来诊的先后次序分为药物治疗组和仪器治疗组，各30例。药物治疗组按各病种的治疗常规投药，仪器治疗组应用奥博脑复康治疗仪，连续治疗 30 天。选用反映脑血流动力学变化的经颅多普勒超声（TCD）和反映脑功能变化的脑电地形图（BEAM）作为观测指标。[结果] 在脑梗塞患者中实验组的疗效明显优于对照组（P﹤0.01），而且 TCD 和 BEAM 在两组间均呈现显著的差异性（P﹤0.05 或﹤0.01）；在脑动脉硬化症患者中实验组的疗效优于对照组（P﹤0.05），而且 TCD 和 BEAM 在两组间均呈现显著的差异性（P﹤0.01）或﹤0.05）；在椎-基底动脉供血不足患者中，实验组的疗效明显优于对照组（P﹤0.05），而且TCD、BEAM 在两组间亦呈现报为显著的差异性（P﹤0.01）。[结论] 应用脑康仪可明显改善脑血流动力学状态，增加脑血流量，强化脑的功能活动，消除脑部病灶周围的组织水肿和肿胀，缓解脑血管痉挛，改善脑组织的血液供给和缺氧状态提高脑组织的新陈代谢，促进病灶同侧或对侧的侧支循环形成，激活处于抑制状态的脑细胞，增强大脑的综合分析能力和记忆功能。可用于治疗脑梗塞、脑动脉硬化症、椎-基底动脉供血不足以及由于强化记忆所致脑功能减退。
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