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1. chinaXiv:202104.00021 [pdf]

“One-to-three” droplet generation-based digital microfluidics for parallel immunoassay.docx

Kai, Jin; Chenxuan, Hu; Siyi, Hu; Jinhua, Li; Hanbin, Ma
Subjects: Biology >> Bioengineering

In digital microfluidics, droplet generation is the fun, as the basis for a series of droplet manipulations including mixing, separation, dispensing, etc., has always been the focus in the field of digital microfluidics. Currently, the performance of DMF-based droplet splitting is highly influenced by the ratio of electrode size and the gap between the upper and lower plates. Here we propose a "one to three" droplet splitting method. In "one to three" spitting method, a pair of opposite electrowetting forces are applied to the droplet. As the electrowetting force is applied on the splitting position, a middle child droplet and two mother liquid droplets generate. The aspect ratio of child droplet is close to 1:2. "One to three" spitting method is then applied to magnetic beads immunoassay. Magnetic beads are retained in child droplets under small magnetic force with high magnetic bead washing efficiency. Simultaneous detection of five B-type natriuretic peptide analyte samples are performed on a single chip within 10 minutes. Correlation coefficient (R2) of the calibration curve was 0.9942, and the detection limit was less than 5pg/mL. This unique method of digital microfluidic droplet splitting has great potential in other application fields.

submitted time 2021-04-07 Hits219Downloads120 Comment 0

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