分类： 生物学 >> 生态学 提交时间： 2024-04-15 合作期刊: 《干旱区科学》
摘要： Land use change affects the balance of organic carbon (C) reserves and the global C cycle. Microbial residues are essential constituents of stable soil organic C (SOC). However, it remains unclear how microbial residue changes over time following afforestation. In this study, 16-, 23-, 52-, and 62-year-old Mongolian pine stands and 16-year-old cropland were studied in the Horqin Sandy Land, China. We analyzed changes in SOC, amino sugar content, and microbial parameters to assess how microbial communities influence soil C transformation and preservation. The results showed that SOC storage increased with stand age in the early stage of afforestation but remained unchanged at about 1.27−1.29 kg/m2 after 52 a. Moreover, there were consistent increases in amino sugars and microbial residues with increasing stand age. As stand age increased from 16 to 62 a, soil pH decreased from 6.84 to 5.71, and the concentration of total amino sugars increased from 178.53 to 509.99 mg/kg. A significant negative correlation between soil pH and the concentration of specific and total amino sugars was observed, indicating that the effects of soil acidification promote amino sugar stabilization during afforestation. In contrast to the Mongolian pine plantation of the same age, the cropland accumulated more SOC and microbial residues because of fertilizer application. Across Mongolian pine plantation with different ages, there was no significant change in calculated contribution of bacterial or fungal residues to SOC, suggesting that fungi were consistently the dominant contributors to SOC with increasing time. Our results indicate that afforestation in the Horqin Sandy Land promotes efficient microbial growth and residue accumulation in SOC stocks and has a consistent positive impact on SOC persistence.
分类： 生物学 >> 生态学 提交时间： 2024-03-13 合作期刊: 《干旱区科学》
摘要： Nitrogen (N) deposition is a significant aspect of global change and poses a threat to terrestrial biodiversity. The impact of plant-soil microbe relationships to N deposition has recently attracted considerable attention. Soil microorganisms have been proven to provide nutrients for specific plant growth, especially in nutrient-poor desert steppe ecosystems. However, the effects of N deposition on plant-soil microbial community interactions in such ecosystems remain poorly understood. To investigate these effects, we conducted a 6-year N-addition field experiment in a Stipa breviflora Griseb. desert steppe in Inner Mongolia Autonomous Region, China. Four N treatment levels (N0, N30, N50, and N100, corresponding to 0, 30, 50, and 100 kg N/(hm2•a), respectively) were applied to simulate atmospheric N deposition. The results showed that N deposition did not significantly affect the aboveground biomass of desert steppe plants. N deposition did not significantly reduce the alfa-diversity of plant and microbial communities in the desert steppe, and low and mediate N additions (N30 and N50) had a promoting effect on them. The variation pattern of plant Shannon index was consistent with that of the soil bacterial Chao1 index. N deposition significantly affected the beta-diversity of plants and soil bacteria, but did not significantly affect fungal communities. In conclusion, N deposition led to co-evolution between desert steppe plants and soil bacterial communities, while fungal communities exhibited strong stability and did not undergo significant changes. These findings help clarify atmospheric N deposition effects on the ecological health and function of the desert steppe.
分类： 生物学 >> 生态学 提交时间： 2024-01-23
摘要： 在基于宏条形码（meta-barcoding）的eDNA监测技术路径中，eDNA测序数据的分析和注释是决定监测结果判断和评估精确与否甚至准确与否的基础，而参考数据库选择、指标阈值选择、目标数据准备是eDNA测序数据分析和注释中最为关键的3个技术环节。为弄清上述3个技术环节处理方案的影响，本研究以长江中游2组eDNA监测COI基因测序数据为分析对象，针对鱼类的检出做了3组实验来分别检验1）不同参考数据库及物种注释算法对注释结果的影响，2）不同OTU聚类序列相似度和物种注释分类置信度（序列一致性和序列覆盖度）对注释结果的影响，3）目标数据中各物种不同序列丰富度对注释结果的影响。结果显示：1）Blast算法下，3个版本nt库注释出的物种基本一致（72%~78%），2个本地序列参考库注释出的物种也基本一致（91%~96%），这5个序列参考库注释出的物种52%~68%一致；nt库RDP Classifier算法注释出的物种覆盖95%以上Blast算法注释出的物种，并比Blast算法注释出的物种多151%~443%，多出的物种大都是错误注释，本地参考数据库RDP Classifier算法注释出的物种覆盖66%~85%的Blast算法注释出的物种，并存在数条只注释到科属的结果。2）OTU聚类序列相似度阈值，取值0.999比取值0.99获得的OTU多154%~209%，注释到鱼类的OTU多240%~490%；注释分类置信度阈值（Blast算法，序列一致性和序列覆盖度）从0.8到0.99注释获得的物种组成基本（94%以上）一致，物种下的OTU组成也基本（83%以上）一致，注释分类置信度阈值取0.7时注释获得的物种组成、OTU组成和取0.8及以上时注释获得的有较大差异。3）在OTU聚类序列相似度阈值0.999、注释分类置信度阈值0.9时，多序列数据注释所得鱼类物种数、OTU数最多、物种注释正确率最高（达81.49%），分别比单序列数据的多7%、215%和高5%。在具体eDNA测序数据的分析和注释中，可通过建立完善本地参考数据库、优化OTU聚类序列相似度和物种注释分类置信度（序列一致性和序列覆盖度）取值、增加目标数据的丰富度来提高注释结果的准确性，但受制于物种注释算法的局限性，物种注释错误和注释遗漏的问题可能将长期存在，物种注释正确率通常低于85%（基于COI基因的eDNA监测）。
分类： 生物学 >> 生态学 提交时间： 2024-01-12 合作期刊: 《干旱区科学》
摘要： Studying the spatiotemporal variations in ecosystem services and their interrelationships on the Loess Plateau against the background of the gully control and land consolidation (GCLC) project has significant implications for ecological protection and quality development of the Yellow River Basin. Therefore, in this study, we took Yan'an City, Shaanxi Province of China, as the study area, selected four typical ecosystem services, including soil conservation service, water yield service, carbon storage service, and habitat quality service, and quantitatively evaluated the spatiotemporal variation characteristics and trade-offs and synergies of ecosystem services from 2010 to 2018 using the Integrated Valuation of Ecosystem Services and Trade-offs (InVEST) model. We also analysed the relationship between the GCLC project and regional ecosystem service changes in various regions (including 1 city, 2 districts, and 10 counties) of Yan'an City and proposed a coordinated development strategy between the GCLC project and the ecological environment. The results showed that, from 2010 to 2018, soil conservation service decreased by 7.76%, while the other three ecosystem services changed relatively little, with water yield service increasing by 0.56% and carbon storage service and habitat quality service decreasing by 0.16% and 0.14%, respectively. The ecological environment of Yan'an City developed in a balanced way between 2010 and 2018, and the four ecosystem services showed synergistic relationships, among which the synergistic relationships between soil conservation service and water yield service and between carbon storage service and habitat quality service were significant. The GCLC project had a negative impact on the ecosystem services of Yan'an City, and the impact on carbon storage service was more significant. This study provides a theoretical basis for the scientific evaluation of the ecological benefits of the GCLC project and the realization of a win-win situation between food security and ecological security.
分类： 生物学 >> 生态学 提交时间： 2024-01-12 合作期刊: 《干旱区科学》
摘要： Regional sustainable development necessitates a holistic understanding of spatiotemporal variations in ecosystem carbon storage (ECS), particularly in ecologically sensitive areas with arid and semi-arid climate. In this study, we calculated the ECS in the Ningxia Section of Yellow River Basin, China from 1985 to 2020 using the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model based on land use data. We further predicted the spatial distribution of ECS in 2050 under four land use scenarios: natural development scenario (NDS), ecological protection scenario (EPS), cultivated land protection scenario (CPS), and urban development scenario (UDS) using the patch-generating land use simulation (PLUS) model, and quantified the influences of natural and human factors on the spatial differentiation of ECS using the geographical detector (Geodetector). Results showed that the total ECS of the study area initially increased from 1985 until reaching a peak at 402.36106 t in 2010, followed by a decreasing trend to 2050. The spatial distribution of ECS was characterized by high values in the eastern and southern parts of the study area, and low values in the western and northern parts. Between 1985 and 2020, land use changes occurred mainly through the expansion of cultivated land, woodland, and construction land at the expense of unused land. The total ECS in 2050 under different land use scenarios (ranked as EPS>CPS>NDS>UDS) would be lower than that in 2020. Nighttime light was the largest contributor to the spatial differentiation of ECS, with soil type and annual mean temperature being the major natural driving factors. Findings of this study could provide guidance on the ecological construction and high-quality development in arid and semi-arid areas.
分类： 生物学 >> 生态学 提交时间： 2024-01-12 合作期刊: 《干旱区科学》
摘要： Gross primary productivity (GPP) of vegetation is an important constituent of the terrestrial carbon sinks and is significantly influenced by drought. Understanding the impact of droughts on different types of vegetation GPP provides insight into the spatiotemporal variation of terrestrial carbon sinks, aiding efforts to mitigate the detrimental effects of climate change. In this study, we utilized the precipitation and temperature data from the Climatic Research Unit, the standardized precipitation evapotranspiration index (SPEI), the standardized precipitation index (SPI), and the simulated vegetation GPP using the eddy covariance-light use efficiency (EC-LUE) model to analyze the spatiotemporal change of GPP and its response to different drought indices in the Mongolian Plateau during 19822018. The main findings indicated that vegetation GPP decreased in 50.53% of the plateau, mainly in its northern and northeastern parts, while it increased in the remaining 49.47% area. Specifically, meadow steppe (78.92%) and deciduous forest (79.46%) witnessed a significant decrease in vegetation GPP, while alpine steppe (75.08%), cropland (76.27%), and sandy vegetation (87.88%) recovered well. Warming aridification areas accounted for 71.39% of the affected areas, while 28.53% of the areas underwent severe aridification, mainly located in the south and central regions. Notably, the warming aridification areas of desert steppe (92.68%) and sandy vegetation (90.24%) were significant. Climate warming was found to amplify the sensitivity of coniferous forest, deciduous forest, meadow steppe, and alpine steppe GPP to drought. Additionally, the drought sensitivity of vegetation GPP in the Mongolian Plateau gradually decreased as altitude increased. The cumulative effect of drought on vegetation GPP persisted for 3.008.00 months. The findings of this study will improve the understanding of how drought influences vegetation in arid and semi-arid areas.
分类： 生物学 >> 生态学 提交时间： 2023-12-20 合作期刊: 《干旱区科学》
摘要： Reclamation of lands abandoned after mining in mountain areas is critical to erosion control, safety from landslides, and ecological protection of mountain ecosystems. However, little is known about alpine coal mine reclamation using the soil seed bank as a potential source for revegetation. We collected samples of persistent soil seed bank for germination experiments from nine reclaimed sites with different soil cover thicknesses and from six control sites in the Qilian Mountains of China. Soil properties of each site were determined (including soil water content, soil available potassium, soil available phosphorus, soil total nitrogen, pH, soil organic matter, soil total phosphorus, and soil total potassium, and soil alkali-hydrolyzable nitrogen), and the relationships of the characteristics of the soil seed bank with soil cover thickness and soil properties were examined. The results showed that the density, number of species, and diversity of the topsoil seed bank were significantly correlated with soil cover thickness, and all increased with the increment of soil cover thickness. Soil cover thickness controlled the soil seed bank by influencing soil properties. With the increase in soil cover thickness, soil properties (e.g., soil organic matter, soil total nitrogen, etc.) content increased while soil pH decreased. The soil seed bank had the potential to restored the pre-mining habitat at reclaimed sites with approximately 20-cm soil cover thickness. Soil properties of reclaimed sites were lower than that of natural sites. The relationship between the soil seed bank and soil cover thickness determined in this study provides a foundation for improving reclamation measures used in coal mines, as well as for the management and monitoring of reclaimed areas.
分类： 生物学 >> 生态学 提交时间： 2023-12-20 合作期刊: 《干旱区科学》
摘要： Grassland degradation can alter the structure and function of ecosystem and soil seed bank. Therefore, estimating the role of soil seed bank in vegetation regeneration of degraded grasslands is crucial. We selected grasslands with three levels of degradation, namely non-degraded (ND), mildly degraded (MD), and heavily degraded (HD) to analyze the effect of grassland degradation on soil seed bank, as well as the role of soil seed bank on vegetation regeneration of the alpine grasslands, China. Soil samples from each level were collected in May, before seedling emergence, in August, after completion of transient seed bank germination, and in December, after seed dispersal, to determine the seed density and species composition through germination experiment. Result showed that a total of 35 plant species was identified, including 15 species observed in both soil seed bank and above-ground vegetation. A total of 19, 15, and 14 species of soil seed bank were identified in December, May, and August, respectively. The most abundant species in soil seed bank were Compositae (5 species), followed by Poaceae (4 species), and Cyperaceae (3 species). Degradation level has no significant impact on species richness and Shannon- Wiener index of soil seed bank. In addition, sampling month and grassland degradation affected soil seed bank density, in which December>May>August, and ND>MD>HD, indicating that density of transient seed bank was greater than persistent seed bank. Soil seed bank density of surface layer (05 cm) accounting for 42%72% of the total density, which was significantly higher than that of deep layer (510 cm). Similarity of species composition between vegetation and soil seed bank was low, and it increased with degradation level (ranged from 0.14 to 0.69). We concluded that grassland degradation affects soil seed bank density more than species diversity, and soil seed bank contributed slightly to vegetation regeneration of degraded alpine grassland. Therefore, it is unlikely that degraded alpine meadow can be restored solely through soil seed bank.
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