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1. chinaXiv:202003.00057 [pdf]

Restoration of brain consciousness ex vivo after circulatory death in pigs

Guo,Zhiyong; Sun,Chengjun; Xu,Guixing; Yin,Meixian; Zhu,Caihui; Zheng,Donghua; Zhang,Yixi; Wang,Linhe; Xie,Rongxing; Zhang,Zhiheng; Gao,Ningxin; Huang,Shanzhou; Yu,Ping; He,Shujiao; Zhao,Qiang; He,Xiaoshun
Subjects: Biology >> Neurobiology

在临床实践中,大脑在心脏停跳数分钟后会出现不可逆的损伤,如果30分钟后患者仍未能成功复苏,将被宣布临床死亡。最新研究显示,在心脏停跳后数小时后,猪的大脑细胞功能仍可在体外得到恢复。然而,在离体状态下意识能否恢复尚不清楚。本研究中,我们建立了用于循环停止后大脑体外复苏的常温机械灌注技术。我们发现大脑单独灌注即能维持大脑循环、细胞结构、代谢活动和脑干功能,但不能恢复大脑意识。而在肝脏的支持下,大脑整体的电活动和意识得以恢复,大脑功能至少可以维持22小时。该技术能够使热缺血50分钟的大脑恢复意识。研究结果表明,肝脏辅助下的大脑常温机械灌注技术可以在循环停止较长时间内恢复和维持大脑的意识。

submitted time 2020-03-23 Hits14763Downloads1762 Comment 0

2. chinaXiv:202003.00008 [pdf]

Germline Polymorphisms and Length of Survival of Nasopharyngeal Carcinoma: An Exome-Wide Association Study in Multiple Cohorts

Yun-Miao Guo; Jie-Rong Chen; Yan-Chun Feng; Chua, Melvin L. K. ; Zeng, Yanni ; Edwin Pun Hui; Allen K. C. Chan; Lin-Quan Tang; Lin Wang; Qian Cui; Hui-Qiong Han; Chun-Ling Luo; Guo-Wang Lin; Yan Liang; Yang Liu; Zhong-Lian He; Yu-Xiang Liu; Pan-Pan Wei; Chu-Jun Liu; Wan Peng
Subjects: Medicine, Pharmacy >> Clinical Medicine

Germline polymorphisms have been linked with differential survival outcomes in cancers but have not been well studied in nasopharyngeal carcinoma (NPC). Here, two-phase association study is conducted to discover germline polymorphisms that are associated with the prognosis of NPC. The discovery phase includes two consecutive hospital cohorts of patients with NPC from Southern China. Exome-wide genotypes at 246,173 single nucleotide polymorphisms (SNPs) are determined, followed by survival analysis for each SNP under Cox proportional hazards regression model. Candidate SNP is replicated in another two independent cohorts from Southern China and Singapore. Meta-analysis of all samples (n = 5,553) confirm that the presence of rs1131636-T, located in the 3′-UTR of RPA1, confers an inferior overall survival (HR = 1.33, 95% CI = 1.20-1.47, P = 6.31 × 10-8). Bioinformatics and biological assays show that rs1131636 has regulatory effects on upstream RPA1. Functional studies further demonstrate that RPA1 promoted the growth, invasion, migration, and radioresistance of NPC cells. Additionally, miR-1253 has been identified as a suppressor for RPA1 expression, likely through regulation of its binding affinity to rs1131636 locus. Collectively, these findings provide a promising biomarker aiding in stratifying patients with poor survival, as well as a potential drug target for NPC.

submitted time 2020-03-06 Hits24567Downloads1481 Comment 0

3. chinaXiv:202002.00074 [pdf]

Molecular karyotypes of loquat (Eriobotrya japonica) aneuploids can be detected by using SSR markers combined with quantitative PCR irrespective of heterozygosity

Wen, Guo; Dang, Jiangbo; Xie, Zhongyi; Wang, Jinying; Jiang, Pengfei; Guo, Qigao; Liang, Guolu
Comment:This article has been published online in Plant Methods (https://plantmethods.biomedcentral.com/articles/10.1186/s13007-020-00568-7)
Subjects: Biology >> Botany
Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Horticulture

Background: Aneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype. Few techniques are currently available for detecting the molecular karyotypes of aneuploids in plants. Methods: By PCR amplification of 209 pairs of SSR primers, 17 pairs of SSR primers with no polymorphism and stable amplification in 23 different ploidy loquat were screened out. Seventeen SSR markers combined with qPCR for detection of aneuploid loquat H39 (2n=2x+5=39) karyotype variation. To verify the method for detection of aneuploid karyotype variation, 9 hybrid offspring of Q24 × ‘Huabai No. 1’ and 16 open-pollination progeny of the triploid loquat A313 and A322 were obtained, and we detected their chromosome numbers using conventional cytological methods. The SSR-qPCR method was used to detect the molecular karyotypes of aneuploids in the 9 hybrid offspring of Q24 × ‘Huabai No. 1’ and 16 open-pollination progeny of the triploid loquat A313 and A322. Results: Based on this imbalance in chromosome dosage, a new approach (referred to as ‘SSR-qPCR’) combining simple sequence repeat (SSR) markers and quantitative real-time PCR (qPCR) has been developed and utilized to detect some common aneuploids irrespective of heterozygosity. We screened 17 specific SSR markers covering all loquat linkage groups and redesigned 6 pairs of primers for SSR markers that can detect loquat chromosome aneuploidies. The SSR-qPCR detection results obtained for hybrid progeny and open-pollination progeny of triploid loquat showed diagnostic accuracies of 88.9% and 62.5%, respectively, compared with the chromosome preparation results. Limitations: Loquat genome sequences have not been published, available SSR markers are currently limited. Conclusion: SSR-qPCR can detect loquat aneuploids and be used to construct the entire molecular karyotypes of aneuploid individuals. Therefore, this method offers a novel alternative for the detection of chromosome aneuploidies.

submitted time 2020-03-03 Hits12169Downloads1226 Comment 0

4. chinaXiv:202002.00066 [pdf]

新型冠状病毒肺炎疫情的动力学分析和预测

诸葛昌靖; 张东艳; 杨武岳; 彭梁榕; 洪柳
Subjects: Mathematics >> Modeling and Simulation

基于经典动力学模型和模型参数自动优化算法, 本文分析了全国当前累计确诊感染病例数大于100(截止到2020年2月16日)的24个省市自治区, 以及湖北省除神农架以外的16个地市从2020年1月20日至2月16日的累计确诊病例数, 并对相应省市地区疫情可能的结束时间和总感染人数的进行了长期预测. 我们的研究表明, 在目前严防严控措施下, 全国大部分省市的疫情将于2月底前基本结束, 而湖北省内疫情也有望于3月中旬结束, 但是武汉市的疫情可能要持续到4月初. 通过对比公开数据与预测值, 我们建议加强对黑龙江、河北、江西、安徽、贵州和四川六省, 以及湖北省内武汉、荆州、鄂州、随州、天门和恩施六个地市的的监控, 以防疫情死灰复燃. 此外, 分析结果提示, 在疫情发展前期, 天津、河北、重庆、四川、海南和广西等省, 以及湖北省下辖多个地市可能存在着聚集性感染, 这有待于疫后进一步的流行病学调查确认.

submitted time 2020-02-25 Hits18108Downloads1689 Comment 0

5. chinaXiv:202002.00069 [pdf]

新冠病毒独立自然连续传播系统的建立及简单模型验证

王基庆; 李志国
Subjects: Physics >> Interdisciplinary Physics and Related Areas of Science and Technology

由于春运出湖北省高峰和全国各地管控开始之间的时间差,使得在这个时间段内,除湖北外的国内地区是个输入一代到感染二代之间独立自然连续传播体系。基于此,我们使用正态分布的线性叠加来拟合全国除湖北外每日新增的数据,简单模型预测和实际新增结果的高度吻合验证了除湖北外是个近似理想系统。拟合计算得到的新冠病毒可再生系数R0=2.2,一代到二代的峰值传播时间是7天。我们还讨论了偏离理想传播体系的原因并分析了一些省的特殊情况。

submitted time 2020-02-25 Hits13904Downloads1391 Comment 0

6. chinaXiv:202002.00009 [pdf]

一种新型冠状病毒肺炎(COVID-19)药物与天然产物快速发现的计算药理学方法

全源; 梁峰吉; 熊江辉
Subjects: Biology >> Virology

新发传染病爆发流行期间,亟需提出候选药物功效与机制的科学假说。疫苗或新药研发均需要一定时间,因而药物重定位(老药新用)策略有其独特价值。但是新发疾病其病原体、宿主反应的临床数据不充分,制约了候选药物假设的提出。此阶段常根据病人临床特征进行广谱抗病毒药物的尝试。本文借鉴人工智能领域常见的启发式搜索思路,提出一种新方法(aCODE),基于前期有一定疗效提示的广谱抗病毒药,获得其宿主靶蛋白集合,在全基因组尺度上搜索与之相关性最高的基因模块组合,进而对候选化合物(如已批准上市药物、天然产物)进行模式匹配与统计检验排序。本方法可根据临床实践的进展更新输入药物,迭代输出更精准结果,输出的天然产物或中药、药食同源成分结合其它信息后可实施快速测试,形成敏捷研发测试闭环。本方法的第二版更新及其与文献证据的比对分析请参考:http://chinaxiv.org/abs/202002.00024。

submitted time 2020-02-21 Hits26949Downloads2678 Comment 0

7. chinaXiv:202002.00033 [pdf]

Decoding evolution and transmissions of novel pneumonia coronavirus using the whole genomic data

Yu, Wen-Bin; Tang, Guang-Da; Zhang, Li; Corlett, Richard T.
Subjects: Biology >> Virology
Subjects: Biology >> Genetics

Background. The outbreak of COVID-19 started in mid-December 2019 in Wuhan, Central China. Up to February 18, 2020, SARS-CoV-2 has infected more than 70,000 people in China, and another 25 countries across five continents. In this study, we used 93 complete genomes of SARS-CoV-2 from the GISAID EpiFluTM database to decode the evolution and human-to-human transmissions of SARS-CoV-2 in the recent two months. Methods. Alignment of coding-regions was conducted haplotype analyses using DnaSP. Substitution sites were analyzed in codon. Evolutionary analysis of haplotypes used NETWORK. Population size changes were estimated using both DnaSP and Arlequin. Expansion date of population size was calculated based on the expansion parameter tau (τ) using the formula t=τ/2u. Findings. Eight coding-regions have 120 substitution sites, including 79 non-synonymous and 40 synonymous substitutions. Forty-two non-synonymous substitutions changed the biochemical property of amino acids. No evident combination was found. Fifty-eight haplotypes were classified as five groups, and 31 haplotypes were found in samples from both China and other countries, respectively. The rooted network suggested H13 and H35 to be ancestral haplotypes, and H1 (and its descendent haplotypes including all samples from the Hua Nan market) was derived H3 haplotype. Population size of SARS-CoV-2 were estimated to have a recent expansion on 6 January 2020, and an early expansion on 8 December 2019. Interpretation. Genomic variations of SARS-CoV-2 are still low in comparisons with published genomes of SARS-CoV and MERS-CoV. Phyloepidemiologic analyses indicated the SARS-CoV-2 source at the Hua Nan market should be imported from other places. The crowded market boosted SARS-CoV-2 rapid circulations in the market and spread it to the whole city in early December 2019. Furthermore, phyloepidemiologic approaches have recovered specific direction of human-to-human transmissions, and the import sources of international infectious cases.

submitted time 2020-02-21 Hits293479Downloads74217 Comment 0

8. chinaXiv:201901.00071 [pdf]

Clinical study on Transcranial magnetoelectric encephalopathy treatment instrument treatmenting parkinson’s disease

Qiang Tang; Wei Zou; Zuodong Sun; Kang Li; Wuyi Sun; Wenhua Wang; Yanli Xing; Xueping Yu; Jing Bai; Xiuying Teng; Yan Hou
Subjects: Medicine, Pharmacy >> Clinical Medicine
Subjects: Physics >> Electromagnetism, Optics, Acoustics, Heat Transfer, Classical Mechanics, and Fluid Dynamics

[Objective] Evaluate on the treatment efficacy and safety for transcranial magnetoelectric encephalopathy treatment instrument (brand name: AOBO Parkinson’s Treatment Instrument) treatmenting parkinson's disease. [Methods] Use methods of double center,randomized, double blind, self crossover, 22 Parkinson’s patients who met the inclusion criteria were randomly divided into A group and B group, then were carried on the curative effect analysis, and were observed therapeutic effect. [Results] The treatment group of 22 cases, basically cured in 0 cases, markedly effective in 9 cases, effective in 8 cases, ineffective in 5 cases. The total efficiency rate and total effective rate were 40.91% (9/22) or 77.27% (17/22) respectively. The control group of 22 cases, basically cured in 0 cases, markedly effective in 2 cases, effective in 3 cases, ineffective in 17 cases. The total efficiency rate and total effective rate were 9.09% (2/22) or 22.73% (5/22) respectively, the total effective rate and total effective rate in the treatment group were higher than those in the control group, the difference was statistically significant (P<0.05). Among them, the main symptoms of resting tremor, rigidity, bradykinesia, evaluation, the treatment group has significant difference (P<0.01); There was no significant difference in the control group (p>0.05); There was significant difference between the treatment group and the control group (p<0.05). [Conclusions] Transcranial magnetoelectric stimulation can significantly improve resting tremor, muscle rigidity, bradykinesia in patients with Parkinson’s disease and other symptoms, and the use of safety. "

submitted time 2019-01-15 Hits23127Downloads1107 Comment 0

9. chinaXiv:201901.00070 [pdf]

重复经颅磁电刺激对海人酸致痫鼠的疗效研究

张旺; 王孝义; 郭洪渠; 宋媛媛; 王爱丽; 孙作东; 沈红
Subjects: Medicine, Pharmacy >> Clinical Medicine
Subjects: Physics >> Electromagnetism, Optics, Acoustics, Heat Transfer, Classical Mechanics, and Fluid Dynamics

目的 探讨经颅磁电刺激(TMES)疗法对海仁酸(KA)致颞叶癫痫鼠的疗效。 方法 62只大鼠按照随机数字表法分为预处理组(共32只)和治疗组(共30只),预处理组再分为4个小组,分别给予治疗仪最大电流强度(MCI) 0%,25%,50%,75%的电流刺激,确定本实验条件下的最佳刺激参数。治疗组再分为3个小组,每组10只。2组(癫痫刺激组、癫痫非刺激组)为符合纳入标准的癫痫模型大鼠,刺激组刺激参数为预处理组确定出的最佳刺激参数;非刺激组大鼠刺激前后处理与刺激组相同,但治疗仪无有效的能量输出。第3组对照组为未造模对照大鼠。3组大鼠均给予每天1次,40 min/次,共14 d的刺激。记录并比较3组大鼠行为学、电生理学及组织学变化,以评估TMES疗法对癫痫大鼠的疗效。 结果 50%MCI为最佳刺激强度。癫痫刺激组大鼠癫痫波出现的频率明显低于癫痫非刺激组大鼠[(30.210 ±4.580)次/min vs. (31.380 ± 4.247)次/min],差异有统计学意义(t=3.235,P=0.001)。Timm染色结果提示3组大鼠染色评分差异有统计学意义(F=17.429, P=0.000),癫痫刺激组大鼠海马齿状回的内分子层Timm染色评分较癫痫非刺激组明显减轻,差异有统计学意义(P<0.05)。结论 TMES疗法可通过改善癫痫大鼠海马齿状回内分子层组织学变化程度来影响齿状回神经元环路的形成,进而降低癫痫脑电波的发作频率。

submitted time 2019-01-02 Hits12903Downloads1150 Comment 0

10. chinaXiv:201812.00135 [pdf]

pCO2在哺乳动物细胞培养中的影响和应用.pdf

刘小双; 徐寒梅
Subjects: Biology >> Bioengineering

二氧化碳分压(pCO2)是哺乳动物细胞培养中一个重要参数,其主要来源包括细胞有氧呼吸产生的CO2、培养基中碳酸氢盐和pH控制加入的CO2。一般细胞培养中不精确控制pCO2,但是将pCO2水平维持在一定范围(30 - 80 mmHg)十分重要,pCO2过高(> 80 mmHg)和过低(< 30 mmHg)不利于细胞生长、代谢、以及蛋白生产和质量控制。大规模高密度细胞培养过程中高pCO2问题比较常见,因此工艺放大时需要考虑不同规模细胞培养中pCO2水平的差异。本文综述了哺乳动物细胞培养中pCO2对细胞生长、代谢和蛋白生产的影响,以及大规模中控制pCO2的方法。

submitted time 2018-12-11 Hits3961Downloads2474 Comment 0

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