Subjects: Medicine, Pharmacy >> Clinical Medicine Subjects: Biology >> Bioengineering Subjects: Physics >> Electromagnetism, Optics, Acoustics, Heat Transfer, Classical Mechanics, and Fluid Dynamics submitted time 2019-01-02
Abstract： Objective To investigate the effects of transcranial magnetoelectric stimulation (TMES) on temporal lobe epilepsy rats induced by kainic acid (KA). Methods 62 rats were divided into pretreatment (32 in total) and treatment (30 in total) groups according to the random number table method. The pretreatment group was further divided into 4 groups, and each group was stimulated by 0 %, 25%, 50%, 75% of the maximum current intensity (MCI) of the therapeutic apparatus respectively. According to the therapeutic efficacy, the optimal stimulation parameters under the experimental conditions was determined. The treatment group was further divided into 3 groups according to the random number table method, 10 in each group. Two groups (epilepsy-stimulating group, epilepsy-non-stimulating group) were epilepsy model rats that met the inclusion criteria. The stimulation parameters in the stimulating group were the best stimulation parameters explored in the pretreatment group; the rats in the non-stimulating group were treated the same before and after stimulation as the stimulating group. However, the therapeutic device has no effective energy output. The rats of third group difined as control were unmodeled control rats. All rats in the three groups were stimulated once a day for 40 minutes each for 14 days. The behavioral, histological and electrophysiological changes in the three groups of rats were recorded and compared to evaluate the efficacy of TMES therapy in epileptic rats. Results 50% MCI is the best stimulus intensity. The frequency of epileptic waves in epilepsy-stimulated rats was significantly lower than that in non-stimulated epileptic rats [(30.210 ± 4.580) beats/min vs. (31.380 ± 4.247) beats/min]. The difference was statistically significant (t = 3.235, P=0.001). The results of Timm staining showed that there was a statistically significant difference in the degree of staining between the three groups (F=17.429, P=0.000). The level of Timm staining in the inner molecular layer of dentate gyrus of the hippocampus in the epilepsy-stimulated group was significantly lower than that in the non-stimulated group, and the difference was statistically significant (P=0.027). Conclusion Transcranial magnetoelectric treatment can influence the formation of dentate gyrus neurons loop by improving epileptic rat dentate gyrus in epileptogenesis in changes of molecular layer organization degree, thereby reducing the frequency of epileptic EEG seizures.
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Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究脱氧雪腐镰刀菌烯醇(DON)和玉米赤霉烯酮(ZEN)对人结直肠腺癌细胞（HCT116细胞）、人肺癌细胞（A549细胞）、人肝癌细胞（HepG2细胞）的促增殖和促迁移作用。设空白对照组，并以赭曲霉毒素A(OTA)作为阳性对照，采用噻唑蓝（MTT）比色法和细胞划痕愈合试验分别检测细胞活力和细胞迁移能力。结果表明：与空白对照组相比，0.080、0.400 μmol/L OTA能显著或极显著增强HCT116、A549和HepG2细胞活力（P<0.05或P<0.01），50.000 μmol/L OTA则能极显著降低HCT116、A549和HepG2细胞活力（P<0.01）；DON在低浓度（0.016、0.080 μmol/L）时对HCT116和A549细胞活力无显著影响（P＞0.05），但能极显著增强HepG2细胞活力（P<0.01），而在DON中高浓度（2.000、10.000、50.000 μmol/L）时则能极显著降低HCT116、A549和HepG2细胞活力（P<0.01）；ZEN浓度在0.016~50.000 μmol/L时能显著或极显著增强HepG2细胞活力（P<0.05或P<0.01），ZEN在高浓度（50.000 μmol/L）时能极显著降低HCT116和A549细胞活力（P<0.01）。细胞划痕愈合试验结果表明，与空白对照组相比，1、10 nmol/L OTA、DON和ZEN作用24、48 h能极显著促进HepG2细胞迁移（P<0.01）。由此可见，在0.016~50.000 μmol/L浓度内，DON和ZEN对HepG2细胞有显著的促进增殖和迁移的作用，表明DON和ZEN在HepG2细胞系上具有潜在的致癌性。
Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在探究地鳖肽对氢化可的松诱导的氧化应激肉仔鸡生长性能、肉品质、脏器指数和抗氧化能力的影响。选用180只1日龄健康爱拔益加（AA）肉仔鸡，随机分为5组，分别为对照组、模型组（25 mg/L氢化可的松）和3个地鳖肽组[0.4、0.8和1.6 g/kg BW，每组6个重复，每个重复6只鸡。从第4天开始，3个地鳖肽组在饮水中加地鳖肽至第21天；从第8天开始，除对照组外，其余各组均在饮水中加氢化可的松，连续5 d。试验期21 d。结果表明：1）14日龄时，模型组肉仔鸡的血清皮质酮含量显著高于对照组和地鳖肽组（P<0.05）。2）1~14日龄时，与对照组相比，模型组肉仔鸡的平均日增重（ADG）显著降低（P<0.05），料重比（F/G）显著增加（P<0.05）；与模型组相比，地鳖肽组的F/G显著降低（P<0.05）。15~21日龄时，模型组的ADG和平均日采食量（ADFI）显著低于对照组（P<0.05）。1~21日龄时，模型组的ADG和ADFI显著低于对照组（P<0.05），F/G显著高于对照组（P<0.05）；与模型组相比，0.4和1.6 g/kg BW地鳖肽组的F/G显著降低（P<0.05）。3）14日龄时，与对照组相比，模型组肉仔鸡的胸肌pH45 min和红度（a*）值显著降低（P<0.05），胸肌、腿肌滴水损失显著升高（P<0.05）；与模型组相比，0.8 g/kg BW地鳖肽组的胸肌pH45 min显著升高（P<0.05），胸肌滴水损失显著降低（P<0.05），0.4和1.6 g/kg BW地鳖肽组的腿肌滴水损失显著降低（P<0.05）。21日龄时，与模型组相比，0.4 g/kg BW地鳖肽组的胸肌a*值显著升高（P<0.05），胸肌滴水损失显著降低（P<0.05）。4）14日龄时，模型组肉仔鸡的脾脏、胸腺和法氏囊指数显著低于对照组（P<0.05）；21日龄时，模型组的法氏囊指数显著低于对照组和1.6 g/kg BW地鳖肽组（P<0.05）。5）14日龄时，模型组肉仔鸡的肝脏和胸肌超氧化物歧化酶（SOD）活性显著低于对照组（P<0.05），肝脏和胸肌谷胱甘肽过氧化物酶（GSH-Px）活性显著低于对照组和0.4 g/kg BW地鳖肽组（P<0.05），肝脏和胸肌过氧化氢酶（CAT）活性显著低于对照组和0.8和1.6 g/kg BW地鳖肽组（P<0.05）。14和21日龄时，模型组的肝脏和胸肌丙二醛（MDA）含量显著高于对照组（P<0.05）。以上结果提示，地鳖肽可有效缓解氧化应激状态下肉仔鸡的应激反应，促进肉仔鸡生长，改善肉品质，提高机体抗氧化能力。
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