Subjects: Biology >> Biophysics >> Biochemistry & Molecular Biology submitted time 2016-05-11
Luo, Huaxia
Sun, Yu
Wei, Guifeng
Luo, Jianjun
Yang, Xinling
Liu, Wei
Chen, Runsheng
Luo, Huaxia
Sun, Yu
Wei, Guifeng
Yang, Xinling
Liu, Wei
Chen, Runsheng
Yang, Xinling
Guo, Mingzhou
Abstract： Long noncoding RNAs (lncRNAs) are pervasively transcribed in the human genome. Recent studies suggest that the involvement of IncRNAs in human diseases could be far more prevalent than previously appreciated. Here we have identified a lncRNA termed Lnc_bc060912 whose expression is increased in human lung and other tumors. Lnc_bc060912 is 1.2 kb in length and is composed of two exons. The expression of Lncbc060912, was repressed by p53. Lnc_bc060912 suppressed cell apoptosis. Using a recently developed method for RNA-pulldown with formaldehyde cross-linking, we found that Lnc_bc060912 interacted With the two DNA damage repair proteins PARP1 and NPM1. Together, these results suggest that Lnc_bc060912, via PARP1 and NPM1, affects cell apoptosis and may play important roles in tumorigenesis and cancer progression.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Biophysics >> Oncology submitted time 2016-05-11
Wei, Guifeng
Luo, Huaxia
Sun, Yu
Tian, Liqing
Liu, Wei
Liu, Lihui
Luo, Jianjun
Chen, Runsheng
Li, Jiagen
He, Jie
Abstract： Esophageal Squamous Cell Carcinoma (ESCC) is among the most common malignant cancers worldwide. In the past, extensive efforts have been made to characterize the involvement of protein-coding genes in ESCC tumorigenesis but few for long noncoding RNAs (lncRNAs). To investigate the transcriptome profile and functional relevance of lncRNAs, we performed an integrative analysis of a customized combined lncRNA-mRNA microarray and RNA-seq data on ESCCs and matched normal tissues. We identified numerous lncRNAs that were differentially expressed between the normal and tumor tissues, termed "ESCC-associated lncRNAs (ESCALs)", of which, the majority displayed restricted expression pattern. Also, a subset of ESCALs appeared to be associated with ESCC patient survival. Gene set enrichment analysis (GSEA) further suggested that over half of the ESCALs were positively- or nelgativelyassociated with metastasis. Among these, we identified a novel nuclear-retained lncRNA, named Epist, which is generally highly expressed in esophagus, and which is down-regulated during ESCC progression. Epist over-expression and knockdown studies further suggest that Epist inhibits the metastasis, acting as a tumor suppressor in ESCC. Collectively, our analysis of the ESCC transcriptome identified the potential tumor suppressing lncRNA Epist, and provided a foundation for future efforts to identify functional lncRNAs for cancerous therapeutic targeting.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biophysics >> Biochemistry & Molecular Biology submitted time 2016-05-11
Abstract： ERp44, an endoplasmic reticulum (ER) resident protein, regulates intracellular Ca2+ release and involves in the maturation of many proteins in mammalian cells. In this study, we investigated the effects and mechanism of ERp44 on cell apoptosis by using ERp44 knockdown stable HeLa cell lines. We found that ERp44 knockdown resulted in increases in cell apoptosis rate more than one fold higher than that of control; using serum starvation, caspase-3 protein level was significantly up-regulated in ERp44 knockdown cells compared to the control cells. Furthermore, we demonstrated that in response to serum starvation, the protein levels of CHOP and GRP78 were also largely raised in ERp44 knockdown cells. Moreover, caspase-12 was activated, which suggested cell apoptosis was induced by ER stress. Taken together, our results indicate that knockdown of ERp44 leads to cell apoptosis through the activation of ER stress. (C) 2015 Elsevier Inc. All rights reserved.
Peer Review Status:Awaiting Review
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