Subjects: Biology >> Bioengineering submitted time 2018-04-19 Cooperative journals: 《中国生物工程杂志》
Abstract:CRISPR/Cas9系统已不仅仅是一种革命性的基因编辑工具,还能在各种原核和真核生物中调控基因转录。近年来,由CRISPR/Cas9衍生而来的CRISPR-dCas9系统已被用于基因成像、高通量筛选、基因调控、必需基因功能研究及表观遗传调控等多个方向。本文总结了近年来CRISPR-dCas9系统在激活或抑制基因转录、降低脱靶效率、sgRNA与转录强度及特异性之间的联系等方面的研究进展,以期对CRISPR-dCas9系统定向调控基因转录的研究提供参考和帮助,并就其未来可能的改进进行了展望。
Subjects: Biology >> Bioengineering submitted time 2017-06-01 Cooperative journals: 《中国生物工程杂志》
Abstract: Metabolic engineering in bacteria requires optimization of gene expression levels to balance metabolic flux distributions and minimize accumulation of toxic intermediates towards an efficient biosynthesis of target products. Small non-coding RNAs (sRNAs) in bacteria can interact with their target mRNA by base-pairing to inhibit or activate the translation of their target genes. Since small RNAs play critical regulatory roles in many physiological processes, they are considered to be powerful tools for the tuning of target genes expression in bacterial metabolic engineering. Many artificial sRNAs are applied in metabolic engineering in recent years. In this review, identification of sRNA targes genes, interaction between sRNAs and their targets and application of sRNAs in metabolic engineering were summarized.