摘要: 为揭示丛枝菌根真菌（AMF）和根瘤菌在白三叶氮（N）同化中的作用，对白三叶进行 单一或者联合接种隐类球囊霉（Paraglomus occultum）和三叶草根瘤菌（Rhizobium trifolii），分 析其对生长、光合作用、叶片N 和氨基酸含量以及N 同化相关酶活性的影响。结果表明：（1） 单一接种AMF 或根瘤菌以及联合接种AMF 和根瘤菌均显著增加了白三叶的株高、匍匐茎长度、 叶片数、地上部生物量、总生物量、叶绿素b 和总叶绿素含量、稳态光量子效率和叶片N 含量， 这种增强效应是联合接种>单一AMF>单一根瘤菌>未接种处理。（2）联合接种AMF 和根瘤菌 还显著增加了白三叶叶片中丙氨酸、精氨酸、天冬酰胺、天冬氨酸、谷氨酰胺、谷氨酸和组氨酸 的含量，同时显著提升了叶片N 同化相关酶如硝酸还原酶、亚硝酸还原酶、谷氨酰胺合成酶、 谷氨酸合成酶、谷氨酸脱氢酶、天冬酰胺合成酶和天冬氨酸转氨酶的活性，且显著促进AMF 对 白三叶根系的侵染。该研究表明了联合接种AMF 和根瘤菌通过激活N 同化相关酶活性有效促进 N 同化，产生更多的氨基酸，进一步促进白三叶植株的生长。文章表明AMF 和根瘤菌具有协同 作用，有效促进了白三叶的N 同化。
Abstract: Symbiotic microorganisms such as arbuscular mycorrhizal fungi (AMF) and rhizobia both promote host plant N acquisition, but whether and how the combination affects host plant nitrogen (N) assimilation remains unclear. To clarify the role of AMF and rhizobia on N assimilation in white clover (Trifolium repens), the effect of single or combined inoculation with an arbuscular mycorrhizal fungus Paraglomus occultum and a rhizobium Rhizobium trifolii on plant growth, photosynthesis, leaf N and amino acid contents, and the activities of N assimilation-related enzymes were analyzed in white clover (Trifolium repens L.) under potted conditions. The results obtained were as follows: (1) The plant height, stolon length, leaf number, shoot biomass, total biomass, chlorophyll b and total chlorothyll contents, steady-state light quantum efficiency, and leaf N contents of white clover were significantly increased by single inoculation with P. occultum, single R. trifolii, and combined inoculations of P. occultum and R. trifolii, along with the order of combined inoculations of P. occultum and R. trifolii > single P. occultum > single R. trifolii > non-inoculation control. (2) Combined inoculations of P. occultum and R. trifolii significantly increased the contents of various amino acids including alanine, arginine, asparagine, aspartate, glutamine, glutamic acid and histidine in leaves of white clover, and distinctly improved the activities of nitroreductase, nitrite reductase, glutamine synthetase, glutamate synthetase, glutamate dehydrogenase, asparagine synthetase, and aspartate aminotransferase. In addition, the introduction of R. trifolii significantly promoted the colonization of P. occultum to roots of white clover, suggesting the partnership between the two. Therefore, it is concluded that combined inoculations of AMF and rhizobia promote N assimilation, generate more amino acids, and further promote the growth of white clover by activating the activities of N assimilation-related enzymes. It is further demonstrated that AMF and rhizobia have synergistic effects on promoting N assimilation of white clover.
| [V1] | 2023-06-05 15:22:58 | ChinaXiv:202306.00037V1 | 下载全文 |
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