摘要: 因具有较强的自由基清除能力和类抗氧化酶特性，氧化铈纳米颗粒（CeO2NPS）已被证明可提高植物的耐 盐性，但其对辣椒种子引发作用和机制尚不明确。为揭示 CeO2 NPS 种子引发处理辣椒对盐胁迫下的萌发及幼苗 生长的影响，以辣椒品种（Capsicum annuum）茂蔬 360 为试验材料，设置了 7 个 CeO2 NPS 浓度（0、0.05、0.1、 0.2、0.3、0.4、0.5 mmolL-1)，以未引发处理组为对照，研究了不同浓度 CeO2NPS 引发处理后对盐胁迫下辣椒 种子萌发、幼苗生物量和生理生化指标的影响。结果表明：（1）0.5 mmolL-1 CeO2NPS 处理后的种子，其可溶 性蛋白质、脯氨酸含量和过氧化氢酶（CAT）活性、还原型抗坏血酸（AsA）含量和 AsA/DHA 比值显著提高， 超氧阴离子(O2 )含量显著降低；盐胁迫下，该处理的种子的发芽率、发芽势、发芽指数、活力指数最大。（2） 0.4 mmolL-1 CeO2NPS 种子引发处理的幼苗在盐胁迫下的鲜重、干重和根长最大，幼苗的可溶性蛋白质、还原 型抗坏血酸（AsA）含量和 AsA/DHA 比值均显著提高。综上认为，CeO2NPS 引发处理可通过降低种子水势、促 进贮藏物质代谢和提高抗氧化能力来提高种子在盐胁迫下的发芽率；同时可在苗期通过增强蛋白合成和抗坏血酸 -谷胱甘肽循环（AsA-GSH）来促进盐胁迫下幼苗的生长。
Abstract: Cerium oxide nanoparticles (CeO2NPS) have been shown to improve the salt tolerance of plant due to the potential to eliminate free radicals and the anti-oxidation properties. However, the effects and mechanisms of seed priming with CeO2NPS on pepper plant are unclear. To reveal impacts of CeO2NPS seed priming on the germination and seedling growth of peeper plants under salt stress, using the Maoshu 360 variety of pepper（Capsicum annuum）as material, a total of 7 concentrations of treatments (0, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5 mmol L-1) and a control of un-treatment were carried out to investigate effects of CeO2NPS seed priming on seed germination rate, biomass, and selected physiological and biochemical indexes under salt stress. The results were as follows: (1) In the seeds primed with 0.5 mmol L-1 cerium oxide nanoparticles, reduced levels of the superoxide anion (O2 ), increased activity of protein synthesis, elevated level of proline as well as increased activity of catalase (CAT), elevated content of ascorbic acid (AsA) and ratio of AsA/DHA were observed. (2)There was no loss of viability for the seeds primed with cerium oxide nanoparticles. Indeed their germination rate and germination potential were enhanced under salt stress, and their germination index, and vigor index were improved under salt stress. (3) Under salt stress, seedlings from seeds primed with 0.4 mmolL-1 cerium oxide nanoparticles shown the highest fresh weight, dry weight, and root length; additionally, soluble protein content, ascorbic acid (AsA) content and AsA/DHA ratio were also improved. In conclusion, seed priming with CeO2NPS could enhance seed germination under salt stress by decreasing seed water potential, accelerating storage substances metabolism and improving antioxidant capacity; also, seed priming with CeO2NPS could promote seedling growth through boosting protein synthesis and ascorbic acid-glutathione cycle (AsA-GSH).
| [V1] | 2023-06-05 15:22:58 | ChinaXiv:202306.00035V1 | 下载全文 |
| 1. Reassessment of Trilophodon connexus Hopwood, 1935 and attributing it to the Choerolophodontidae | 2023-09-20 |
| 2. MSPD-HPLC 法分析云南红豆杉中的10-DAB Ⅲ和紫杉醇 | 2023-09-20 |
| 3. 广西植物名录补遗（Ⅷ） | 2023-09-20 |
| 4. 白及根腐病病原菌的鉴定及抑制效应研究 | 2023-09-20 |
| 5. 虎耳草属的资源分类及育种进展 | 2023-09-20 |
| 6. 木论喀斯特常绿落叶阔叶林空间结构动态研究 | 2023-09-20 |
| 7. 锦绣杜鹃花药发育及散粉孔形成的形态与解剖学研究 | 2023-09-20 |
| 8. 基于叶绿体基因组解析半枫荷系统位置和进化 | 2023-09-20 |
| 9. Reappraisal of Bothriolepis sinensis Chi, 1940 from the Tiaomachien Formation, Hunan, China | 2023-09-05 |
| 10. 鄯善库木塔格沙漠北缘细菌群落结构特征及影响因素 | 2023-08-26 |
相关信息
点击量
下载量
