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OsIMA1 增强水稻对镉逆境的适应性

摘要:铁(Fe)是植物生长发育所必需的营养元素而镉(Cd)是对植物有害的元素,且植 物对 Fe 和 Cd 的吸收存在拮抗作用。OsIMA 是一类正调控水稻 Fe 吸收的一类小肽,其过表 达可以促进 Fe 的积累。为探究 OsIMA 是否参与水稻对 Cd 胁迫的适应性,该研究以水稻为 研究材料,利用荧光定量PCR 分析了OsIMA 基因的表达水平,通过遗传转化和CRISPR/Cas9 基因编辑技术构建了 OsIMA1 过表达植物和 ima1 突变体植物,评估了 OsIMA1 过表达和突 变体植物在 Cd 逆境条件下的株高,并用利用电联耦合等离子体质谱法测量了根和地上部的 Fe 和 Cd 含量。结果表明:(1)Cd 处理后,OsIMA1 和 OsIMA2 的转录水平上调。(2)OsIMA1 过表达植物比野生型植物对 Cd 胁迫更耐受。(3)ima1 功能缺失突变体比野生型植物对 Cd 胁迫更敏感。(4)Cd 含量检测发现,OsIMA1 过表达植株根系的 Cd 含量较高,而 ima1 突 变体植株地上部的 Cd 含量较高。综上所述,OsIMA1 通过限制 Cd 从根向地上部的转运以 增强水稻对 Cd 逆境的适应能力,该研究为定向培育耐 Cd 作物提供了理论依据。

英文摘要:Iron (Fe) is crucial for the growth and development of plants and cadmium (Cd) is toxic to plants. There is an antagonistical mechanism between Fe and Cd uptake in plants. OsIMAs are a class of small peptides, and their overexpression improves Fe accumulation in rice. To explore the role of OsIMA genes in response to Cd stress, we analyzed the expression of two OsIMA genes by qRT-PCR, generated OsIMA1 overexpression plants and CRISPR/Cas9 edited ima1 mutants by genetic transformation, assessed the phenotypes of OsIMA1 overexpressing plants and ima1 loss-of-function mutant plants under Cd stress, and measured the Fe and Cd concentration of root and shoot. The results were as follows: (1) Both OsIMA1 and OsIMA2 were induced by Cd treatment. (2) Overexpression of OsIMA1 gene improved the tolerance of plants to Cd stress. (3) The loss-of-function of OsIMA1 led to the higher sensitivity of plants to Cd stress. (4) Measurement of Cd concentration indicated that OsIMA1 overexpressing plants accumulated more Cd in the root and the ima1 mutants accumulated more Cd in the shoot. Taken together, these data suggest that OsIMA1 improves Cd tolerance by restricting Cd translocation from root to shoot, which provides the theoretical base for breeding the Cd-tolerant rice.

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[V1] 2022-06-29 20:52:10 chinaXiv:202206.00189V1 下载全文
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