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婆婆纳抗黑色素瘤物质基础的初步研究

Abstracts

为明确婆婆纳(Veronica didyma Tenore)抗黑色素瘤活性部位及物质基础,该文采用 CCK8 法评价了婆婆纳乙醇提取物四个萃取部位(石油醚层、乙酸乙酯层、正丁醇层、水层)、 乙醇提取物及单体化合物对黑色素瘤细胞株(B16 和A375)细胞的增殖抑制作用;利用植 物化学的技术和方法对活性部位的化学成分进行了系统分离纯化研究。结果表明:(1)乙 酸乙酯萃取部位(PPNE)较其他样品有更好的抑制B16 和A375 细胞增殖的作用,其IC50 值分别为0.177 mg·mL-1(B16)、2.826 mg·mL-1(A375)。(2)从活性部位PPNE 中得到 7 个单体化合物,即对羟基苯甲醛(1)、胡黄连苷II(2)、isoscutellarein 7 - O - (6''' - Oacetyl) - β - allopyranosyl (1'''→2'') - β - glucopyranoside(3)、3' - hydroxyl - 4' - O - methylisoscutellarein 7 - O - [6''' - O - acetyl - β - D - allopyranosyl - (1→2) - β - D - Glucopyranoside(4)、6 - O - veratroylcatalposide(5)、Veronicoside(6)、isoscutellarein 4' - methyl ether 7 - O - (6''' - O - acetyl) - ballopyranosyl (1'''→2'') - β - glucopyranoside(7),7 个化合物均为首次从该植物中 分离得到,HPLC 分析显示这7 个化合物是PPNE 的较大量成分。除化合物1 外,其他6 个 单体化合物均有较好的抑制黑色素瘤细胞增殖的作用,该文首次报道了化合物3、4、7 的抗 黑色素瘤活性。婆婆纳的乙酸乙酯萃取部位(PPNE)为其抗黑色素瘤活性部位,环烯醚萜 类化合物2、5、6 和黄酮类化合物3、4、7 可能为PPNE 抗黑色素瘤的活性基础。该研究结 果为合理利用其资源奠定了科学基础。
[英文摘要]To clarify the anti-melanoma active extract and material basis of Veronica didyma Tenore, CCK8 method was used to evaluate the inhibitory effects of four extracts (petroleum ether extract, ethyl acetate extract, n-butanol extract, water extract), ethanol extract and monomer compounds on the proliferation of melanoma cell lines (B16 and A375); The chemical constituents of the active fraction were systematically separated and purified by phytochemical techniques and methods. The results were as follows: (1) Ethyl acetate extract (PPNE) had better inhibitory effect on the proliferation of B16 and A375 cells than other samples, and IC50 = 0.177 mg·mL-1 (B16), IC50 = 2.826 mg·mL-1 (A375), respectively. Seven monomers were obtained from PPNE were p-hydroxybenzaldehyde (1), Picroside II (2), isoscutellarein 7 - O - (6''' - Oacetyl) - β - allopyranosyl (1'''→2'') - β - glucopyranoside (3), 3' - hydroxyl - 4' - O - methylisoscutellarein 7 - O - [6''' - O - acetyl - β - D - allopyranosyl - (1→2) - β - D - Glucopyranoside (4), 6 - O - veratroylcatalposide (5), Veronicoside (6), isoscutellarein 4' - methyl ether 7 - O - (6''' - O - acetyl) - ballopyranosyl (1'''→2'') - β - glucopyranoside (7). (2) Seven compounds were isolated from this plant for the first time, and HPLC showed that these seven compounds are the major components of PPNE. Except Compound 1, the other six monomers all have good inhibitory effect on the proliferation of melanoma cells, and the anti-melanoma activities of compounds 3, 4 and 7 are reported for the first time. Based on these results, PPNE of Veronica didyma Tenore is an anti melanoma active fraction, Iridoids (compounds 2, 5, 6) and flavonoids (compounds 3, 4, 7) may be the basis of anti melanoma activity of PPNE. The results of this study will lay scientific foundation for the rational use of Veronica didyma Tenore sresources.
Download Comment Cooperative journals:《广西植物》 Hits:4123 Downloads:360
Recommended references: 刘芳,张远芳,李玲环,彭忠禄,王俊杰.(2021).婆婆纳抗黑色素瘤物质基础的初步研究.广西植物.[ChinaXiv:202112.00133] (Click&Copy)
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[V1] 2021-12-19 21:30:36 chinaXiv:202112.00133V1 Download
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