Abstract:
The H3 histone variant CENP-A is an epigenetic marker critical for the centromere identity and function. However, the precise regulation of the spatiotemporal deposition and propagation of CENP-A at centromeres during the cell cycle is still poorly understood. Here, we show that CENP-A is phosphorylated at Ser68 during early mitosis by Cdk1. Our results demonstrate that phosphorylation of Ser68 eliminates the binding of CENP-A to the assembly factor HJURP, thus preventing the premature loading of CENP-A to the centromere prior to mitotic exit. Because Cdk1 activity is at its minimum at the mitotic exit, the ratio of Cdk1/PP1 alpha activity changes in favor of Ser68 dephosphorylation, thus making CENP-A available for centromeric deposition by HJURP. Thus, we reveal that dynamic phosphorylation of CENP-A Ser68 orchestrates the spatiotemporal assembly of newly synthesized CENP-A at active centromeres during the cell cycle.
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Journal:
DEVELOPMENTAL CELL
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Subject:
Biology
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Biophysics
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Cell Biology
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Cite as:
ChinaXiv:201605.01469
(or this version
ChinaXiv:201605.01469V1)
DOI:10.12074/201605.01469V1
CSTR:32003.36.ChinaXiv.201605.01469.V1
- Recommended references:
Yu, Zhouliang,Zhou, Xiang,Wang, Wenjing,Deng, Wenqiang,Fang, Junnan,Hu, Hao,Wang, Zichen,Cui, Lei,Shen, Jing,Ou, Guangshuo,Yang, Na,Chen, Ping,Xu, Rui-Ming,Li, Guohong,Yu, Zhouliang,Deng, Wenqiang,Fang, Junnan,Hu, Hao,Peng, Shengyi,Li, Shangze,Zhang, Xiaodong,Cui, Lei,Lou, Jizhong,Zhai, Linhui,Xu, Ping,Peng, Shengyi,Yuan, Zengqiang,Wong, Jiemin,Wong, Jiemin,Dong, Shuo.(2016).Dynamic Phosphorylation of CENP-A at Ser68 Orchestrates Its Cell-Cycle-Dependent Deposition at Centromeres.DEVELOPMENTAL CELL.[ChinaXiv:201605.01469]
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